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Overview

Uniprot IDA0A480K104
Protein NameATP-dependent 6-phosphofructokinase
Gene NamePFKL
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
280 PITSRYVKDLVVQRL
392 YKLLAHQKISKEKTN
677 FDRNYGTKLGVKAIL
715 CVIGLQKKAVAFSPV

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway

Protein Sequence

10 MATVDLEKLR 20 TSGAGKAIGV 30 LTSGGDAQGM 40 NAAVRAVTRM 50 GIYVGAKVFL 60 ICEGYEGLVE 70 GGENIKPANW 80 LSVSNIIQLG 90 GTVIGSARCK 100 AFTTREGRLA 110 AAYNLVQRGI 120 TNLCVIGGDG 130 SLTGANIFRS 140 EWGSLLAELV 150 SEGRISESTA 160 QTYSHLSIAG 170 LVGSIDNDFC 180 GTDMTIGTDS 190 ALHRIMEVID 200 AITTTAQSHQ 210 RTFVLEVMGR 220 HCGYLALVSA 230 LASGADWLFI 240 PEAPPEDGWE 250 NFMCERLGET 260 RSRGSRLNII 270 IIAEGAIDRH 280 GKPITSRYVK 290 DLVVQRLGFD 300 TRVTVLGHVQ 310 RGGTPSAFDR 320 VLSSKMGMEA 330 VMALLEATPD 340 TPACVVSLSG 350 NQSVRLPLME 360 CVQMTKEVQK 370 AMDEKRFDEA 380 IQLRGRSFEN 390 NWNIYKLLAH 400 QKISKEKTNF 410 SLAILNVGAP 420 AAGMNAAVRS 430 AVRSGISQGH 440 TVYVVHDGFE 450 GLAKGQVQEV 460 GWHDVAGWLG 470 RGGSMLGTKR 480 TLPKAYLEKI 490 VENIRTYNIH 500 ALLVIGGFEA 510 YEGVLQLVEA 520 RGRYEELCIV 530 MCVIPATISN 540 NVPGTDFSLG 550 SDTAVNAAME 560 SCDRIKQSAS 570 GTKRRVFIVE 580 TMGGYCGYLA 590 TVTGIAVGAD 600 AAYVFEDPFN 610 IQDLKANVEH 620 MTEKMKTDIQ 630 RGLVLRNEKC 640 HEYYTTEFLY 650 NLYSSEGRGI 660 FDCRTNVLGH 670 LQQGGAPTPF 680 DRNYGTKLGV 690 KAILWMSEKL 700 RAVYRKGRVF 710 ANAPDSACVI 720 GLQKKAVAFS 730 PVTELKEDTD 740 FEHRMPREQW 750 WLNLRLMLKM 760 LAHYRISMAD 770 YVSGELEHVT 780 RRTLSIETGF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005945 6-phosphofructokinase complex
Cellular Component GO:0016020 membrane
Molecular Function GO:0003872 6-phosphofructokinase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0070095 fructose-6-phosphate binding
Molecular Function GO:0046872 metal ion binding
Biological Process GO:0061621 canonical glycolysis
Biological Process GO:0030388 fructose 1,6-bisphosphate metabolic process
Biological Process GO:0006002 fructose 6-phosphate metabolic process

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.