Overview
| Uniprot ID | A0A480VU46 |
|---|
| Protein Name | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 |
|---|
| Gene Name | RPN1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 219 |
RNVESYTKLGNPTRS |
| 548 |
VSTLNSGKKSLELEH |
| 549 |
STLNSGKKSLELEHK |
| 556 |
KSLELEHKALTSEVA |
| 570 |
ALLQSRLKTEGSDLC |
| 591 |
QKLDAQVKELVLKSA |
| 596 |
QVKELVLKSAVEAER |
| 611 |
LVAGKLKKDTYIENE |
| 619 |
DTYIENEKLISGKRQ |
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity
Protein Sequence
10
MLADPMVNCV
20
VAEAGRRGGL
30
AGGLRLSSIP
40
AMEAPAVCLL
50
PLLLLLWAWA
60
PAPGRASPEA
70
LPLVNEDVKR
80
TVDLSSHLAK
90
VTAEVVLAHA
100
GSSSSPRAAS
110
FLLALEPELE
120
ARLAHLGVQV
130
KGEDEEENNL
140
EVRETKIKGK
150
SGRFFTVTLP
160
VALDPGAKIS
170
VTVETVYTHV
180
LQPYPTQITQ
190
SEKQFVVFEG
200
NHYFYSPYPT
210
KSQSMRVKLA
220
SRNVESYTKL
230
GNPTRSEDLL
240
DYGPFRDVPP
250
YSQDTFKVHS
260
ENNSPFLTIT
270
SMTRVIEVSH
280
WGNIAVEENV
290
DLKHTGAVLK
300
GPFSRYDYQR
310
QPDSGISSIR
320
SFKTILPAAA
330
QDVYYRDEIG
340
NVSTSHLLIL
350
DDSVEMEIRP
360
RFPLFGGWKT
370
HYIVGYNLPS
380
YEYLYNLGDQ
390
YALKMRLVDH
400
VFDEQVIDSL
410
TVKIILPEGA
420
KNIQVDSPYE
430
ISRAPDELHY
440
TYLDTFGRPV
450
IVAHKKNLVE
460
QHIQDIVVHY
470
TFNKVLMLQE
480
PLLVVAAFYI
490
LFFTVIVYVR
500
LDFSITKDPA
510
AEARMKVACI
520
TEQVLTLVNK
530
RIGLYRHFDE
540
TINRYKQSRD
550
VSTLNSGKKS
560
LELEHKALTS
570
EVALLQSRLK
580
TEGSDLCDKV
590
SEMQKLDAQV
600
KELVLKSAVE
610
AERLVAGKLK
620
KDTYIENEKL
630
ISGKRQELVT
KIDHILDAL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0008250 |
oligosaccharyltransferase complex |
| Cellular Component |
GO:0160226 |
oligosaccharyltransferase complex A |
| Cellular Component |
GO:0160227 |
oligosaccharyltransferase complex B |
| Cellular Component |
GO:0005791 |
rough endoplasmic reticulum |
| Molecular Function |
GO:0016740 |
transferase activity |
| Biological Process |
GO:0006487 |
protein N-linked glycosylation |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.