Search Results
Overview
| Uniprot ID | A0A4X1T4X9 |
|---|---|
| Protein Name | Dihydrofolate reductase |
| Gene Name | DHFR |
| Organism | Sus scrofa |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 107 | LTEQPELKNKVDMVW |
| 33 | PPLRNEYKYFQRMTT |
Function
Catalyzes the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate in a NADPH-dependent manner. Key enzyme in folate metabolism. Contributes to the nuclear and mitochondrial de novo thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2
Protein Sequence
10
MVRPLNCIVA
20
VSQNMGIGKN
30
GDLPWPPLRN
40
EYKYFQRMTT
50
TSSVEGKQNL
60
VIMGRKTWFS
70
IPEKNRPLKD
80
RINIVLSREL
90
KEPPQGAHFL
100
AKSLDDALKL
110
TEQPELKNKV
120
DMVWIVGGSS
VYKMFRSSF
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Molecular Function | GO:0004146 | dihydrofolate reductase activity |
| Molecular Function | GO:0050661 | NADP binding |
| Biological Process | GO:0006730 | one-carbon metabolic process |
| Biological Process | GO:0031427 | response to methotrexate |
| Biological Process | GO:0046654 | tetrahydrofolate biosynthetic process |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.