Overview
| Uniprot ID | A0A4X1T8R5 |
|---|
| Protein Name | Eukaryotic translation initiation factor 3 subunit H |
|---|
| Gene Name | EIF3H |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 153 |
PKLMEVCKEKDFSPE |
| 155 |
LMEVCKEKDFSPEAL |
| 163 |
DFSPEALKKGNITFE |
| 202 |
KKSAVADKHELLSLA |
| 281 |
EDLSKLFKPHQPPAR |
| 5 |
***MASRKEGTGSAA |
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression
Protein Sequence
10
MASRKEGTGS
20
AATSSSSTTG
30
AAGKGKGKGG
40
SGDSAVKQVQ
50
IDGLVVLKII
60
KHYQEEGQGT
70
EHTEDDADFD
80
EVQYQMEMMR
90
SLRHVNIDHL
100
HVGWYQSTYY
110
GSFVTRALLD
120
SQFSYQHAIE
130
ESVVLIYDPI
140
KTAQGSLSLK
150
AYRLTPKLME
160
VCKEKDFSPE
170
ALKKGNITFE
180
HMFEEVPIVI
190
KNSHLINVLM
200
WELEKKSAVA
210
DKHELLSLAS
220
SNHLGKNLQL
230
LMDRVDEMSQ
240
DIVKYNTYLR
250
NTSKQQQQKH
260
QYQQRRQQEN
270
MQRQSRGEPP
280
LPEEDLSKLF
290
KPHQPPARMD
300
SLLIAGQINT
310
YCQNIKEFTA
320
QNLGKLFMAQ
ALQEYNN
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0016282 |
eukaryotic 43S preinitiation complex |
| Cellular Component |
GO:0033290 |
eukaryotic 48S preinitiation complex |
| Cellular Component |
GO:0005852 |
eukaryotic translation initiation factor 3 complex |
| Molecular Function |
GO:0008237 |
metallopeptidase activity |
| Molecular Function |
GO:0003743 |
translation initiation factor activity |
| Biological Process |
GO:0001732 |
formation of cytoplasmic translation initiation complex |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.