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Overview

Uniprot IDA0A4X1TEM7
Protein NamePyruvate kinase
Gene NamePKM
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
110 SRSVETLKEMIKSGM
114 ETLKEMIKSGMNVAR
137 EYHAETIKNVRAATE
163 VAVALDTKGPEIRTG
183 GTAEVELKKGATLKI
214 LDYKNICKVVDVGSK
255 GGFLGSKKGVNLPGA
295 VFASFIRKAADVHEV
309 VRKVLGEKGKNIKII
314 GEKGKNIKIISKIEN
318 KNIKIISKIENHEGV
441 EAAMFHRKLFEELVR
546 NLAMNVGKARGFFKK

Function

Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival

Protein Sequence

10 MALRKILLQG 20 LRLKNKAPWT 30 GHSMCCHQWI 40 CPASTAGLSS 50 SGPKTSEAMP 60 KPHSDAGTAF 70 IQTQQLHAAM 80 ADTFLEHMCR 90 LDIDSPPITA 100 RNTGIICTIG 110 PASRSVETLK 120 EMIKSGMNVA 130 RLNFSHGTHE 140 YHAETIKNVR 150 AATESFASDP 160 ILYRPVAVAL 170 DTKGPEIRTG 180 LIKGSGTAEV 190 ELKKGATLKI 200 TLDNAYMEKC 210 DENVLWLDYK 220 NICKVVDVGS 230 KVYVDDGLIS 240 LLVKQKGPDF 250 LVTEVENGGF 260 LGSKKGVNLP 270 GAAVDLPAVS 280 EKDIQDLKFG 290 VEQDVDMVFA 300 SFIRKAADVH 310 EVRKVLGEKG 320 KNIKIISKIE 330 NHEGVRRFDE 340 ILEASDGIMV 350 ARGDLGIEIP 360 AEKVFLAQKM 370 MIGRCNRAGK 380 PVICATQMLE 390 SMIKKPRPTR 400 AEGSDVANAV 410 LDGADCIMLS 420 GETAKGDYPL 430 EAVRMQHLIA 440 REAEAAMFHR 450 KLFEELVRAS 460 SHSTDLMEAM 470 AMGSVEASYK 480 CLAAALIVLT 490 ESGRSAHQVA 500 RYRPRAPIIA 510 VTRNHQTARQ 520 AHLYRGIFPV 530 VCKDPVQEAW 540 AEDVDLRVNL 550 AMNVGKARGF 560 FKKGDVVIVL 570 TGWRPGSGFT NTMRVVPVP

Gene Ontology

Classification GO ID Description
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016301 kinase activity
Molecular Function GO:0000287 magnesium ion binding
Molecular Function GO:0030955 potassium ion binding
Molecular Function GO:0004743 pyruvate kinase activity

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.