Overview
| Uniprot ID | A0A4X1TGU9 |
|---|
| Protein Name | Eukaryotic translation initiation factor 3 subunit E |
|---|
| Gene Name | EIF3E |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 265 |
TTAVITNKDVRKRRQ |
| 35 |
VKEIYNEKELLQGKL |
| 383 |
RNARLDAKIDSKLGH |
| 409 |
QQVIEKTKSLSFRSQ |
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins
Protein Sequence
10
MAEYDLTTRI
20
AHFLDRHLVF
30
PLLEFLSVKE
40
IYNEKELLQG
50
KLDLLSDTNM
60
VDFAMDVYKN
70
LYSDDIPHAL
80
REKRTTVVAQ
90
LKQLQAETEP
100
IVKMFEDPET
110
TRQMQSTRDG
120
RMLFDYLADK
130
HGFRQEYLDT
140
LYRYAKFQYE
150
CGNYSGAAEY
160
LYFFRVLVPA
170
TDRNALSSLW
180
GKLASEILMQ
190
NWDAAMEDLT
200
RLKETIDNNS
210
VSSPLQSLQQ
220
RTWLIHWSLF
230
VFFNHPKGRD
240
NIIDLFLYQP
250
QYLNAIQTMC
260
PHILRYLTTA
270
VITNKDVRKR
280
RQVLKDLVKV
290
IQQESYTYKD
300
PITEFVECLY
310
VNFDFDGAQK
320
KLRECESVLV
330
NDFFLVACLE
340
DFIENARLFI
350
FETFCRIHQC
360
ISINMLADKL
370
NMTPEEAERW
380
IVNLIRNARL
390
DAKIDSKLGH
400
VVMGNNAVSP
410
YQQVIEKTKS
420
LSFRSQMLAM
430
NIEKKLNQNS
440
RSEAPNWATQ
DSGFY
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0016282 |
eukaryotic 43S preinitiation complex |
| Cellular Component |
GO:0033290 |
eukaryotic 48S preinitiation complex |
| Cellular Component |
GO:0071540 |
eukaryotic translation initiation factor 3 complex, eIF3e |
| Cellular Component |
GO:0016605 |
PML body |
| Molecular Function |
GO:0003743 |
translation initiation factor activity |
| Biological Process |
GO:0001732 |
formation of cytoplasmic translation initiation complex |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.