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Overview

Uniprot IDA0A4X1TSN2
Protein NameTrifunctional enzyme subunit beta, mitochondrial
Gene NameHADHB
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
202 QRLSLISKFRLNFLS
409 NYMGRKAKVGSPPLE

Function

Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity

Protein Sequence

10 MTSLLTYTFK 20 SLPSTSKWAL 30 RFSLRPLSSS 40 SQLQAAAAVQ 50 TKSKKTLAKP 60 NIRNIVVVDG 70 VRIPFLQSGT 80 SYKELMPHDL 90 ARAALLGLLH 100 RTSVPKDVVD 110 YLVFGTVIQE 120 VKTSNVAREA 130 ALGAGFSDKT 140 PAHTVTMACI 150 SSNQAMTTGV 160 SLIASGQFDV 170 VVAGGVELMS 180 DIPIRHSRKM 190 RKMMLDLNKA 200 KTLGQRLSLI 210 SKFRLNFLSP 220 ELPAVAEFST 230 SETMGHSADR 240 LAAAFAVSRS 250 EQDEYALRSH 260 SLAKKAQDEG 270 LLSDVVPFKV 280 PGKDTVTQDN 290 GIRPSSLEQM 300 AKLKPAFIKP 310 YGTVTAANSS 320 FLTDGASAVL 330 IMAEEKALAM 340 GYKPKAYLRD 350 FTYVSQDPKD 360 QLLLGPTYAT 370 PKVLEKAGLT 380 MNDIDAFEFH 390 EAFSGQILAN 400 LKAMDSDWFA 410 QNYMGRKAKV 420 GSPPLEKFNN 430 WGGSLSLGHP 440 FGATGCRLVM 450 TAANRLRKEG 460 GQYGLVAACA 470 AGGQGHAMIV EAYPK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0016507 mitochondrial fatty acid beta-oxidation multienzyme complex
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005741 mitochondrial outer membrane
Molecular Function GO:0003985 acetyl-CoA C-acetyltransferase activity
Molecular Function GO:0050633 acetyl-CoA C-myristoyltransferase activity
Biological Process GO:0006635 fatty acid beta-oxidation

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.