Overview
| Uniprot ID | A0A5G2QES7 |
|---|
| Protein Name | S-(hydroxymethyl)glutathione dehydrogenase |
|---|
| Gene Name | ADH5 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 121 |
CKFCLNPKTNLCQKI |
| 127 |
PKTNLCQKIRVTQGK |
| 240 |
IIGVDINKDKFARAK |
Function
Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Class-III ADH is remarkably ineffective in oxidizing ethanol. Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage. Also acts as a S-nitroso-glutathione reductase by catalyzing the NADH-dependent reduction of S-nitrosoglutathione, thereby regulating protein S-nitrosylation
Protein Sequence
10
VKESRCKQTT
20
RSLFSPFQVI
30
KCKAAVAWEA
40
GKPLSIEEIE
50
VAPPKAHEVR
60
IKIIATAVCH
70
TDAYTLSGAD
80
PEGSFPVILG
90
HEGAGIVESV
100
GEGVTKLKAG
110
DTVIPLYIPQ
120
CGECKFCLNP
130
KTNLCQKIRV
140
TQGKGLMPDG
150
TSRFTCKGKT
160
ILHYMGTSTF
170
SEYTVVADIS
180
VAKIDPLAPL
190
DKVCLLGCGV
200
STGYGAAVNT
210
AKVEPGSTCA
220
VFGLGGVGLA
230
VIMGCKVAGA
240
SRIIGVDINK
250
DKFARAKEFG
260
ASECINPQDF
270
SKPIQEVLIE
280
MTDGGVDYSF
290
ECIGNVKVMR
300
AALEACHKGW
310
GVSVVVGVAA
320
SGEEIATRPF
330
QLVTGRTWKG
340
TAFGGWKSVE
350
SIPKLVSEYM
360
SKKIKVDEFV
370
THNLPFDQIN
380
EAFELMHAGK
SIRTVIKL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Molecular Function |
GO:0004022 |
alcohol dehydrogenase (NAD+) activity |
| Molecular Function |
GO:0106322 |
S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity |
| Molecular Function |
GO:0106321 |
S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity |
| Molecular Function |
GO:0080007 |
S-nitrosoglutathione reductase (NADH) activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0046294 |
formaldehyde catabolic process |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.