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Overview

Uniprot IDA0A5G2QGH4
Protein NameC-1-tetrahydrofolate synthase, cytoplasmic
Gene NameMTHFD1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
256 DVAYSEAKERAGFIT
348 LYGETKAKVLLSSLE
482 PSVNGVRKFSDIQLR
537 TNDRFLRKITIGQAP
547 IGQAPTEKGHSRTAQ
55 NVKLKAAKEIGIKAM
678 MEKFFNIKCRYSGLR
769 DLVSRLAKEHGAFDA
872 LSHNPEQKGVPTGFV

Function

Trifunctional enzyme that catalyzes the interconversion of three forms of one-carbon-substituted tetrahydrofolate: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate, 5,10-methenyltetrahydrofolate and (6S)-10-formyltetrahydrofolate. These derivatives of tetrahydrofolate are differentially required in nucleotide and amino acid biosynthesis, (6S)-10-formyltetrahydrofolate being required for purine biosynthesis while (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate is used for serine and methionine biosynthesis for instance

Protein Sequence

10 MGLPVIILQI 20 REKLKSKVAQ 30 MKEQVPGFTP 40 GLAILQVGNR 50 DDSNLYINVK 60 LKAAKEIGIK 70 AMHIKLPRTA 80 TESEVLKCIT 90 SLNEDLTVHG 100 FILQLPLDSE 110 NPINAEALIN 120 AIAPEKDVDG 130 LTSINAGKLA 140 RGDLNDCFIP 150 CTPKGCLELI 160 KQTGVQIAGR 170 QAVVIGRSKI 180 VGAPMHDLLL 190 WNHATVTTCH 200 SKTANLNEEV 210 NKGDIVVVAA 220 GKPEMVKGEW 230 IKPGAVVIDC 240 GINCVPDDTK 250 PSGKRIVGDV 260 AYSEAKERAG 270 FITPVPGGVG 280 PMTVAMLMQS 290 TVESAEHFLE 300 KFRPGKWIIQ 310 YNKLNLKTPV 320 PSDIDISRSC 330 KPKPIGNLAR 340 EIGLLSEEVE 350 LYGETKAKVL 360 LSSLERLKHQ 370 PDGKYVVVTG 380 ITPTPLGEGK 390 STTTIGLVQA 400 LGAHLHQNVF 410 ACVRQPSQGP 420 TFGIKGGAAG 430 GGYSQVIPME 440 EFNLHLTGDI 450 HAITAANNLV 460 AAAIDARMFH 470 EQTQTDKALF 480 NRLVPSVNGV 490 RKFSDIQLRR 500 LWRLGIEKTD 510 PTTLTDEEIN 520 RFARLDIDPE 530 TITWQRVLDT 540 NDRFLRKITI 550 GQAPTEKGHS 560 RTAQFDISVA 570 SEIMAVLALT 580 SSLEDMRERL 590 SKMVVASSKK 600 GEPISAEDLG 610 VSGALTVLMK 620 DAIKPNLMQT 630 LEGTPVFVHA 640 GPFANIAHGN 650 SSIIADRIAL 660 KLVGPEGFVV 670 TEAGFGADIG 680 MEKFFNIKCR 690 YSGLRPHVVV 700 LVATVRALKM 710 HGGGPTVTAG 720 LPLPKAYIEE 730 NLELVEKGFS 740 NLRKQIENAK 750 MFGVPVVVAV 760 NAFKTDTEAE 770 LDLVSRLAKE 780 HGAFDAVKCT 790 HWAEGGKGAL 800 ALAQAVQRAA 810 QAPSSFQLLY 820 DLELPIEDKI 830 RIIAQKIYGA 840 DDIELLPEAQ 850 HKAEVYTKQG 860 FGNLPICMAK 870 THLSLSHNPE 880 QKGVPTGFVL 890 PIRDIRASVG 900 AGFLYPLVGT 910 MSTMPGLPTR 920 PCFYDIDLDP ETEQVNGLF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004329 formate-tetrahydrofolate ligase activity
Molecular Function GO:0004477 methenyltetrahydrofolate cyclohydrolase activity
Molecular Function GO:0004488 methylenetetrahydrofolate dehydrogenase (NADP+) activity
Biological Process GO:0035999 tetrahydrofolate interconversion

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.