Search Results

Overview

Uniprot IDA0A5G2QHF3
Protein NameDNA replication licensing factor MCM5
Gene NameMCM5
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
196 EGYALPRKCNTDQAG
256 CDRYLCDKVVPGNRV
391 GAKSQLLKFVEKCSP
395 QLLKFVEKCSPRVYT
54 DRTGFTFKYRDELKR

Function

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity

Protein Sequence

10 MSGFDDPGIF 20 YSDSFGGDNA 30 ADEGQARKSQ 40 LQRRFKEFLR 50 QYRVGTDRTG 60 FTFKYRDELK 70 RHYNLGEYWI 80 EVEMEDLASF 90 DEDLADYLYK 100 QPAEHLQLLE 110 EAAKEVADEV 120 TRPRPTGDEV 130 LQDIQVMLKS 140 DASPSSIRSL 150 KSDTMSHLVK 160 IPGIVIAASG 170 VRAKATRISI 180 QCRSCRNTLT 190 NIAMRPGLEG 200 YALPRKCNTD 210 QAGRPKCPLD 220 PYFIMPDKCK 230 CVDFQTLKLQ 240 ELPDAVPHGE 250 MPRHMQLYCD 260 RYLCDKVVPG 270 NRVTIMGIYS 280 IKKFGLTSNR 290 GRDRVGVGIR 300 SAYIRVLGIQ 310 VDTDGSGRSF 320 AGAVTPQEEE 330 EFRRLAALPN 340 VYEVVSKSIA 350 PSIFGGTDMK 360 KAIACLLFGG 370 SRKRLPDGLT 380 RRGDINLLML 390 GDPGAKSQLL 400 KFVEKCSPRV 410 YTSGKGSSAA 420 GLTASVMRDP 430 SSRNFIMEGG 440 AMVLADGGVV 450 CIDEFDKMRE 460 DDRVAIHEAM 470 EQQTISIAKA 480 GITTTLNSRC 490 SVLAAANSVF 500 GRWDETKGED 510 NIDFMPTILS 520 RFDMIFIVKD 530 EHNEERDVML 540 AKHVITLHVS 550 ALTQTQAVEG 560 EIDLAKLKKF 570 IAYCRAKCGP 580 RLSAEAAEKL 590 KNRYIIMRSG 600 ARQHERDSDR 610 RSSIPITVRQ 620 LEAIVRIAEA 630 LSKMKLQPFA 640 TEADVEEALR 650 LFQVSTLDAA 660 LSGTLSGVEG 670 FTSQEDQELL 680 SRIEKQLKRR 690 FAIGSQVSEH 700 SIIQDFTKQK 710 YPEHAIHRVL 720 QLMLRRGELQ 730 HRMQRKVLYR LK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0071162 CMG complex
Cellular Component GO:0042555 MCM complex
Cellular Component GO:0005634 nucleus
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0003678 DNA helicase activity
Molecular Function GO:0003688 DNA replication origin binding
Molecular Function GO:0016787 hydrolase activity
Molecular Function GO:0003697 single-stranded DNA binding
Biological Process GO:0006270 DNA replication initiation
Biological Process GO:0000727 double-strand break repair via break-induced replication

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.