Search Results

Overview

Uniprot IDA0A5G2QJD9
Protein NameAP2-associated protein kinase 1
Gene NameAAK1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
169 VARLHQCKTPIIHRD
371 IAPRQRPKAGQTQPN

Function

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Preferentially, may phosphorylate substrates on threonine residues. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity

Protein Sequence

10 MKKFFDSRRE 20 QGGSGLGSGS 30 SGGGGSTSGL 40 GSGYIGRVFG 50 IGRQQVTVDE 60 VLAEGGFAIV 70 FLVRTSNGMK 80 CALKRMFVNN 90 EHDLQVCKRE 100 IQIMRDLSGH 110 KNIVGYIDSS 120 INNVSSGDVW 130 EVLILMDFCR 140 GGQVVNLMNQ 150 RLQTGFTESE 160 VLQIFCDTCE 170 AVARLHQCKT 180 PIIHRDLKVE 190 NILLHDRGHY 200 VLCDFGSATN 210 KFQNPQTEGV 220 NAVEDEIKKY 230 TTLSYRAPEM 240 VNLYSGKIIT 250 TKADIWALGC 260 LLYKLCYFTL 270 PFGESQVAIC 280 DGNFTIPDNS 290 RYSQDMHCLI 300 RYMLEPDPDK 310 RPDIYQVSFF 320 SFKLLKKECP 330 VPNVQNSPIP 340 AKLPEPVKAS 350 EAAAKKTQPK 360 ARLTDPIPTT 370 ETSIAPRQRP 380 KAGQTQPNPG 390 ILPIQPALTP 400 RKRAMVQPPP 410 QVAGSSNQPG 420 LLASVPQPKT 430 QAPPSQPLPQ 440 SQAKQPQAPP 450 APQQPPSAPA 460 QGLPAQAQAT 470 PQHQQQLFLK 480 QQPQPPQPQP 490 QAQAPPAAQQ 500 PSGTFYQQQQ 510 QQQQQQAQAQ 520 QFQAVHPAAQ 530 QPAIAQFPAV 540 SQGSSQQQLI 550 QNFYQQQQQQ 560 QQQQQLATTL 570 HQQQLLTQQA 580 ALQQKTTVAA 590 IQPPQAQPAT 600 ASQPPPAQEP 610 AIQAPVRQQP 620 KVQTTPPPAI 630 QGQKLGSLTP 640 PSSPKAQRAG 650 HRRILSDVTH 660 SAVFGVPASK 670 STQLLQAAAA 680 EASLNKSKSA 690 TTTPSGSPRA 700 SQQNVYNPSE 710 GSTWNPFDDD 720 NFSKLTAEEL 730 LNKDFAKLGE 740 GKHPEKLGGS 750 AESLIPGFQP 760 TQGDAFAASS 770 FAAGTAEKRK 780 GGQAVDSSLP 790 LLSVSDPFIP 800 LQVPDAPEKL 810 IEGLKSPDTS 820 LLLPDLLPMT 830 DPFGSTSDAV 840 IEKADVAVES 850 LIPGLEPPVP 860 QRLPSQTESV 870 ASNRTDSLTG 880 EDALIDCSLL 890 SNPTTDLLEE 900 FAPIAISAPA 910 HKAAEDSNLI 920 SGFDVPEGSD 930 KVAEDEFDPI 940 PVLITKNPQG 950 LQREPNPCPV 960 ITIEHFKETA 970 GVKGLPLYPD 980 PSRGPGPKTQ 990 NSLESDYLAR 1000 DGPSSNSSFH 1010 SSEEEGTDLE 1020 GDMLDCSGSR 1030 PLLMESEEED 1040 ESCKPLQGKL 1050 GGAVPFAQPE 1060 GSTEPAKAAQ 1070 GGRKNQFRAL 1080 TQPTADGLGE 1090 PDVFATAPFR 1100 SSRVPTDDTD 1110 IFAKAPFVSK 1120 GSVALSQPEE 1130 ADVFLRAPFT 1140 KKKSLEELAG 1150 AQIATSQESP 1160 AQAGLLGPTD 1170 DVPLLPGLDR 1180 AVYPSVRAQY 1190 SVAGFAQQSN 1200 LPSHSVQAAD 1210 RLDSISPRGP 1220 ALESGAHPND 1230 RNKGPQPQKE 1240 VVSGPVAGKP 1250 FRPQSLSKYS 1260 RHYSPEDEPT 1270 PEAQPIAAYK 1280 IVSQTNKQSI 1290 AGSVSITSLS 1300 SRTTELPAAD 1310 PFALAPFPSK SGKQKP

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005905 clathrin-coated pit
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0098793 presynapse
Molecular Function GO:0035612 AP-2 adaptor complex binding
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0005112 Notch binding
Molecular Function GO:0004674 protein serine/threonine kinase activity
Biological Process GO:0045747 positive regulation of Notch signaling pathway
Biological Process GO:0140238 presynaptic endocytosis
Biological Process GO:0050821 protein stabilization
Biological Process GO:2000369 regulation of clathrin-dependent endocytosis
Biological Process GO:0032880 regulation of protein localization

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.