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Overview

Uniprot IDA0A5G2QKH4
Protein NameLeucine--tRNA ligase, cytoplasmic
Gene NameLARS1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
103 PIKACADKLKREIEL
1056 RRVPVLGKEHTEKTP
137 KTEDIILKDKAKGKK
194 EYTLIKLKVLEPYPC
202 VLEPYPCKLSGLKGK
464 DYGEEKWKEQTCQCL
472 EQTCQCLKNLETFCE
888 HFETSSGKLPDNKVI
893 SGKLPDNKVIASELG
906 LGNLPELKKYMKKVM
925 MIKENLEKTGPRVLD
970 FASEAEDKVREDCCP
979 REDCCPGKPLNVFRT

Function

Aminoacyl-tRNA synthetase that catalyzes the specific attachment of leucine to its cognate tRNA (tRNA(Leu)). It performs tRNA aminoacylation in a two-step reaction: Leu is initially activated by ATP to form a leucyl-adenylate (Leu-AMP) intermediate; then the leucyl moiety is transferred to the acceptor 3' end of the tRNA to yield leucyl-tRNA. To improve the fidelity of catalytic reactions, it is also able to hydrolyze misactivated aminoacyl-adenylate intermediates (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing)

Protein Sequence

10 MAERKGTTKV 20 DFLKKIEKEI 30 QQKWDTEKVF 40 EVNASDLEKQ 50 TSKDKYFVTF 60 PYPYMNGRLH 70 LGHTFSLSKC 80 EFAVGYQRLK 90 GKTCLYPFGL 100 HCTGMPIKAC 110 ADKLKREIEL 120 YGCPPDFPDE 130 EEEEEEINAK 140 TEDIILKDKA 150 KGKKVDWRRS 160 FITTDVNPYY 170 DSFVRWQFLT 180 LRERNKIKFG 190 KGVGPQEYTL 200 IKLKVLEPYP 210 CKLSGLKGKN 220 VFLVAATLRP 230 ETMFGQTNCW 240 VHPEIKYIGF 250 ETVNGDIFIC 260 TPRAARNMSY 270 QGFTKDNGVV 280 PVVKELMGEE 290 ILGASLSAPL 300 TSYKVIYVLP 310 MLTIKEDKGT 320 GVVTSVPSDS 330 PDDFAAFRDL 340 KKKQALRAKY 350 GIRDDMVLPF 360 EPVPIIEIPG 370 FGKLSAVTLC 380 DELKVQSQND 390 REKLAEAKEK 400 LYLKGFYDGI 410 MLVDGFEGQK 420 VQDVKKSIRN 430 RMIDTGDAYA 440 YMEPEKQVMS 450 RSSDECVVAL 460 CDQWYLDYGE 470 EKWKEQTCQC 480 LKNLETFCEE 490 TRRNFEATLD 500 WLQEHACSRT 510 YGLGTRLPWD 520 EQWLIESLSD 530 STIYMAFYTA 540 AHLLQGGDIR 550 GQAESPLGIR 560 PQQMTKEVWD 570 YIFFKEAPFP 580 KTQIPKEKLD 590 QLKQEFEFWY 600 PVDLRVSGKD 610 LIPNHLSYFL 620 YNHVAMWPEE 630 SDKWPVAVRA 640 NGHLLLNSEK 650 MSKSTGNFLT 660 LTEALDKYSA 670 DGMRLALADA 680 GDTVEDANFV 690 EAMADAGVLR 700 LYTWVEWVKE 710 MVANWDSLRS 720 GPANTFNDRV 730 FASEMNAGII 740 KTDQNYKMMF 750 KEALKTGFFE 760 FQAAKDKYRE 770 LAIEGMHRDL 780 VFRFIEVQTL 790 LLAPFCPHLC 800 EHIWTLLGKP 810 DSIMNASWPV 820 AGPVDESLIR 830 SSQYLMEVAH 840 DLRLRLKNYM 850 TPAKGKKPDK 860 QPPQKPSHCT 870 IYVAKDYPSW 880 QHVTLSVLRS 890 HFETSSGKLP 900 DNKVIASELG 910 NLPELKKYMK 920 KVMPFVAMIK 930 ENLEKTGPRV 940 LDLQLEFDEQ 950 AVLMENIVYL 960 TNSLELERIE 970 VKFASEAEDK 980 VREDCCPGKP 990 LNVFRTEPGV 1000 LVSLVNPQPF 1010 SGHFSAKIEI 1020 RQGDNRDSVI 1030 RRLMKVDRGI 1040 KDLSKVKLMR 1050 FDDPLLGPRR 1060 VPVLGKEHTE 1070 KTPISEQAVF 1080 HVDLTSKKVH 1090 LTENGLRADI GDTMIYLVH

Gene Ontology

Classification GO ID Description
Cellular Component GO:0017101 aminoacyl-tRNA synthetase multienzyme complex
Cellular Component GO:0005737 cytoplasm
Molecular Function GO:0002161 aminoacyl-tRNA deacylase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004823 leucine-tRNA ligase activity
Biological Process GO:0006429 leucyl-tRNA aminoacylation

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.