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Overview

Uniprot IDA0A5G2QN33
Protein NameAP2-associated protein kinase 1
Gene NameAAK1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
169 VARLHQCKTPIIHRD
371 IAPRQRPKAGQTQPN

Function

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Preferentially, may phosphorylate substrates on threonine residues. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity

Protein Sequence

10 MKKFFDSRRE 20 QGGSGLGSGS 30 SGGGGSTSGL 40 GSGYIGRVFG 50 IGRQQVTVDE 60 VLAEGGFAIV 70 FLVRTSNGMK 80 CALKRMFVNN 90 EHDLQVCKRE 100 IQIMRDLSGH 110 KNIVGYIDSS 120 INNVSSGDVW 130 EVLILMDFCR 140 GGQVVNLMNQ 150 RLQTGFTESE 160 VLQIFCDTCE 170 AVARLHQCKT 180 PIIHRDLKVE 190 NILLHDRGHY 200 VLCDFGSATN 210 KFQNPQTEGV 220 NAVEDEIKKY 230 TTLSYRAPEM 240 VNLYSGKIIT 250 TKADIWALGC 260 LLYKLCYFTL 270 PFGESQVAIC 280 DGNFTIPDNS 290 RYSQDMHCLI 300 RYMLEPDPDK 310 RPDIYQVSFF 320 SFKLLKKECP 330 VPNVQNSPIP 340 AKLPEPVKAS 350 EAAAKKTQPK 360 ARLTDPIPTT 370 ETSIAPRQRP 380 KAGQTQPNPG 390 ILPIQPALTP 400 RKRAMVQPPP 410 QVAGSSNQPG 420 LLASVPQPKT 430 QAPPSQPLPQ 440 SQAKQPQAPP 450 APQQPPSAPA 460 QGLPAQAQAT 470 PQHQQQLFLK 480 QQPQPPQPQP 490 QAQAPPAAQQ 500 PSGTFYQQQQ 510 QQQQQQAQAQ 520 QFQAVHPAAQ 530 QPAIAQFPAV 540 SQGSSQQQLI 550 QNFYQQQQQQ 560 QQQQQLATTL 570 HQQQLLTQQA 580 ALQQKTTVAA 590 IQPPQAQPAT 600 ASQPPPAQEP 610 AIQAPVRQQP 620 KVQTTPPPAI 630 QGQKLGSLTP 640 PSSPKAQRAG 650 HRRILSDVTH 660 SAVFGVPASK 670 STQLLQAAAA 680 EASLNKSKSA 690 TTTPSGSPRA 700 SQQNVYNPSE 710 GSTWNPFDDD 720 NFSKLTAEEL 730 LNKDFAKLGE 740 GKHPEKLGGS 750 AESLIPGFQP 760 TQGDAFAASS 770 FAAGTEKADV 780 AVESLIPGLE 790 PPVPQRLPSQ 800 TESVASNRTG 810 LQREPNPCPV 820 ITIEHFKETA 830 GVKGLPLYPD 840 PSRGPGPKTQ 850 NSLESDYLAR 860 DGPSSNSSFH 870 SSEEEGTDLE 880 GDMLDCSGSR 890 PLLMESEEED 900 ESCKPLQGKL 910 GGAVPFAQPE 920 GSTEPAKAAQ 930 GGRKNQFRAL 940 TQPTADGLGE 950 PDVFATAPFR 960 SSRVPTDDTD 970 IFAKAPFVSK 980 GSVALSQPEE 990 ADVFLRAPFT 1000 KKKSLEELAG 1010 AQIATSQESP 1020 AQAGLLGPTD 1030 DVPLLPGLDR 1040 AVYPSVRAQY 1050 SVAGFAQQSN 1060 LPSHSVQAAD 1070 RLDSISPRGP 1080 ALESGAHPND 1090 RNKGPQPQKE 1100 VVSGPVAGKP 1110 FRPQSLSKYS 1120 RHYSPEDEPT 1130 PEAQPIAAYK 1140 IVSQTNKQSI 1150 AGSVSITSLS 1160 SRTTELPAAD 1170 PFALAPFPSK SGKQKP

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005905 clathrin-coated pit
Cellular Component GO:0098793 presynapse
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004674 protein serine/threonine kinase activity
Biological Process GO:0006897 endocytosis

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.