Overview
| Uniprot ID | A0A5G2QNM9 |
|---|
| Protein Name | Acyl-protein thioesterase 1 |
|---|
| Gene Name | LYPLA1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 263 |
SLTVERLKTLVNPAN |
Function
Acts as an acyl-protein thioesterase. Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acts as a palmitoyl thioesterase that catalyzes depalmitoylation of proteins, such as ADRB2, KCNMA1 and SQSTM1. Acts as a negative regulator of autophagy by mediating palmitoylation of SQSTM1, decreasing affinity between SQSTM1 and ATG8 proteins and recruitment of ubiquitinated cargo proteins to autophagosomes. Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS). Has much higher thioesterase activity than lysophospholipase activity. Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA
Protein Sequence
10
MRARGPRGQP
20
GPPGAGWPDI
30
VTRAAGGADG
40
EARARGPGGR
50
RGGGAHAPER
60
APVRGSDSPF
70
ARPWAAAGSR
80
SAFRFRRAVC
90
RAGVCAAITC
100
RRLCPPSCPP
110
PGRPPPRHGW
120
AEAFAGIRSA
130
HIKYICPHAP
140
VMPVTLNMNM
150
AMPSWFNIIG
160
LSPDSLEDET
170
GIKQAAENVK
180
SLIEQEVKNG
190
IPSNRIILGG
200
FSQGGALSLY
210
TALTTHQKLA
220
GVTALSCWLP
230
LRSSFPECPI
240
SGVNRDIPIL
250
QCHGVLDPLV
260
PLMFGSLTVE
270
RLKTLVNPAN
280
VTFKTYEGMM
290
HSSCQQEMLD
300
VKQFIEKLLP
PID
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005783 |
endoplasmic reticulum |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0031965 |
nuclear membrane |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:0008474 |
palmitoyl-(protein) hydrolase activity |
| Molecular Function |
GO:0004622 |
phosphatidylcholine lysophospholipase A1 activity |
| Biological Process |
GO:0006631 |
fatty acid metabolic process |
| Biological Process |
GO:0015908 |
fatty acid transport |
| Biological Process |
GO:1905336 |
negative regulation of aggrephagy |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.