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Overview

Uniprot ID	A0A5G2QPT0
Protein Name	AP2-associated protein kinase 1
Gene Name	AAK1
Organism	Sus scrofa

Kla Sites from experimental identification

Position	Flanking peptide
169	VARLHQCKTPIIHRD
371	IAPRQRPKAGQTQPN

Function

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Preferentially, may phosphorylate substrates on threonine residues. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity

Protein Sequence

10 MKKFFDSRRE 20 QGGSGLGSGS 30 SGGGGSTSGL 40 GSGYIGRVFG 50 IGRQQVTVDE 60 VLAEGGFAIV 70 FLVRTSNGMK 80 CALKRMFVNN 90 EHDLQVCKRE 100 IQIMRDLSGH 110 KNIVGYIDSS 120 INNVSSGDVW 130 EVLILMDFCR 140 GGQVVNLMNQ 150 RLQTGFTESE 160 VLQIFCDTCE 170 AVARLHQCKT 180 PIIHRDLKVE 190 NILLHDRGHY 200 VLCDFGSATN 210 KFQNPQTEGV 220 NAVEDEIKKY 230 TTLSYRAPEM 240 VNLYSGKIIT 250 TKADIWALGC 260 LLYKLCYFTL 270 PFGESQVAIC 280 DGNFTIPDNS 290 RYSQDMHCLI 300 RYMLEPDPDK 310 RPDIYQVSFF 320 SFKLLKKECP 330 VPNVQNSPIP 340 AKLPEPVKAS 350 EAAAKKTQPK 360 ARLTDPIPTT 370 ETSIAPRQRP 380 KAGQTQPNPG 390 ILPIQPALTP 400 RKRAMVQPPP 410 QVAGSSNQPG 420 LLASVPQPKT 430 QAPPSQPLPQ 440 SQAKQPQAPP 450 APQQPPSAPA 460 QGLPAQAQAT 470 PQHQQQLFLK 480 QQPQPPQPQP 490 QAFKVKSLKF 500 YPFYPMCKGS 510 PCPYLQLESF 520 QAVHPAAQQP 530 AIAQFPAVSQ 540 GSSQQQLIQN 550 FYQQQQQQQQ 560 QQQLATTLHQ 570 QQLLTQQAAL 580 QQKTTVAAIQ 590 PPQAQPATAS 600 QPPPAQEPAQ 610 IQAPVRQQPK 620 VQTTPPPAIQ 630 GQKLGSLTPP 640 SSPKAQRAGH 650 RRILSDVTHS 660 AVFGVPASKS 670 TQLLQAAAAE 680 ASLNKSKSAT 690 TTPSGSPRAS 700 QQNVYNPSEG 710 STWNPFDDDN 720 FSKLTAEELL 730 NKDFAKLGEG 740 KHPEKLGGSA 750 ESLIPGFQPT 760 QGDAFAASSF 770 AAGTAEKRKG 780 GQAVDSSLPL 790 LSVSDPFIPL 800 QVPDAPEKLI 810 EGLKSPDTSL 820 LLPDLLPMTD 830 PFGSTSDAVI 840 EKADVAVESL 850 IPGLEPPVPQ 860 RLPSQTESVA 870 SNRTDSLTGE 880 DALIDCSLLS 890 NPTTDLLEEF 900 APIAISAPAH 910 KAAEDSNLIS 920 GFDVPEGSDK 930 VAEDEFDPIP 940 VLITKNPQGG 950 HSRNSSGSSE 960 SSLPNLARSL LLVDQLIDL

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005905	clathrin-coated pit
Cellular Component	GO:0098793	presynapse
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0004674	protein serine/threonine kinase activity
Biological Process	GO:0006897	endocytosis

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.