Overview
| Uniprot ID | A0A5G2QQE8 |
|---|
| Protein Name | Apoptosis-inducing factor 1, mitochondrial |
|---|
| Gene Name | AIFM1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 252 |
VAVLTGKKVVQLDVR |
Function
Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Binds to DNA in a sequence-independent manner. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import
Protein Sequence
10
MISSISVTIV
20
RKFANPCSRS
30
VVNTRSITGR
40
EGAETFLLHL
50
KVPASSRRRK
60
SMKLTCNLFQ
70
RWHVPLELQM
80
TRQMASSGAS
90
GGKIDNTVLV
100
LFVGLSTIGA
110
GAYAYRTIKD
120
NKKRYNERIA
130
GLGLTPEEKQ
140
KRATSSAPEG
150
EPVPQVRVPS
160
HVPFLLIGGG
170
TAAFAAARSI
180
RARDPGARVL
190
IVSEDPELPY
200
MRPPLSKELW
210
FSDDPNVTKT
220
LRFRQWNGKE
230
RSIYFQPPSF
240
YVSAQDLPHI
250
ENGGVAVLTG
260
KKVVQLDVRG
270
NMAKLNDGSQ
280
ITYEKCLIAT
290
GGTPRSLSAI
300
DRAGAEVKSR
310
TTLFRKIGDF
320
RTLEKISREV
330
KSITIIGGGF
340
LGSELACALG
350
RKARALGTEV
360
IQLFPEKGNM
370
GKILPEYLSN
380
WTMEKVRREG
390
VKVLPNAIVQ
400
SVGVSGGKLL
410
IKLKDGRKVE
420
TDHIVAAVGL
430
EPNVELAKTG
440
GLEIDSDFGG
450
FRVNAELQAR
460
SNIWVAGDAA
470
CFYDIKLGRR
480
RVEHHDHAVV
490
SGRLAGENMT
500
GAAKPYWHQS
510
MFWSDLGPDV
520
GYEAIGLVDS
530
SLPTVGVFAK
540
ATAQDNPKSA
550
TEQSGTGIRS
560
ESETESEAPE
570
ITLPVGSPAV
580
PQAPTQGEDY
590
GKGVIFYLRD
600
KVVVGIVLWN
610
VFNRMPIARK
620
IIKDGEQHED
630
LNEVAKLFNI
HED
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005743 |
mitochondrial inner membrane |
| Cellular Component |
GO:0005758 |
mitochondrial intermembrane space |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0071949 |
FAD binding |
| Molecular Function |
GO:0016174 |
NAD(P)H oxidase H2O2-forming activity |
| Molecular Function |
GO:0046983 |
protein dimerization activity |
| Biological Process |
GO:0006915 |
apoptotic process |
| Biological Process |
GO:0033108 |
mitochondrial respiratory chain complex assembly |
| Biological Process |
GO:0043065 |
positive regulation of apoptotic process |
| Biological Process |
GO:0045041 |
protein import into mitochondrial intermembrane space |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.