Search Results

Overview

Uniprot ID	A0A5G2QYY1
Protein Name	Monofunctional C1-tetrahydrofolate synthase, mitochondrial
Gene Name	MTHFD1L
Organism	Sus scrofa

Kla Sites from experimental identification

Position	Flanking peptide
276	RWKLHCLKLQPLSPV
296	ISRAQTPKAVDVLAK
458	NGIREFSKIQLARLK

Function

May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism complementing thus the enzymatic activities of MTHFD2

Protein Sequence

10 SDSFALKVIQ 20 NSKEVLSLLQ 30 EKNPAFKPVL 40 AVIQAGEDNL 50 MQEINQKLAE 60 EAGLNITHIC 70 LPADSSEDEI 80 IDEILKINED 90 SRVHGLALQI 100 SETSFSSKIL 110 HALKPEKDVD 120 GVTDVNLGKL 130 VRGDAHDCFV 140 SPVARAVIEL 150 LEKSGVNLDG 160 RKILVIGAHG 170 SLEATLQCLF 180 QRKGSMTMSS 190 QWKAPQLQSK 200 LHEADVVIFG 210 SPKPEEVPLS 220 WIQQGTAVLN 230 CSHDFLSGKW 240 LQIGCQATEN 250 NRSNWSSLWV 260 QQNMVGSGRR 270 WLREQQYRRW 280 KLHCLKLQPL 290 SPVPSDIEIS 300 RAQTPKAVDV 310 LAKEIGLLED 320 EIEIYGRSKA 330 KVRLSLLERL 340 KDQADGKYVL 350 VAGITPTPLG 360 EGKSTVTIGL 370 VQAMTAHLNV 380 NSFACLRQPS 390 QGPTFGVKGG 400 AAGGGYAQVI 410 PMEEFNLHLT 420 GDIHAITAAN 430 NLLAAAIDTR 440 ILHENTQTDK 450 ALYNRLVPSV 460 NGIREFSKIQ 470 LARLKKLGIN 480 KTDPSALTED 490 EMRKFARLDI 500 DPATITWQRV 510 LDTNDRFLRK 520 ITLGRGTQRK 530 AQFDIAVASE 540 IMAVLALTDS 550 LKDMKERLGR 560 MVVASDKNGQ 570 PVTADDLGVT 580 GALTVLMKDA 590 IKPNLMQTLE 600 GTPVFVHAGP 610 FANIAHGNSS 620 VLADKIALKL 630 VGEEGFVVTE 640 AGFGADIGME 650 KFFNIKCRAS 660 GLVPNVVVLV 670 ATVRALKMHG 680 GGPSVTAGVP 690 LKKEYTEENL 700 QLVADGCCNL 710 KKQIQIAQLF 720 GVPVVVALNV 730 FKTDTRAEID 740 LVCELAKQAG 750 AFDAVPCYHW 760 SVGGKGSVDL 770 AWAVSEAAHK 780 GSRFQFLYDV 790 QLPIVEKIRT 800 IAQAVYGARD 810 IELSSEAQSK 820 IDRYTEQGFG 830 NLPICMAKTH 840 LSLSHQPDKK 850 GVPKDFILPI 860 TDVRASIGAG 870 FIYPLVGTMS 880 TMPGLPTRPC 890 FYDIDLDTET EQVQGLF

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005759	mitochondrial matrix
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0004329	formate-tetrahydrofolate ligase activity
Molecular Function	GO:0004488	methylenetetrahydrofolate dehydrogenase (NADP+) activity
Biological Process	GO:0046394	carboxylic acid biosynthetic process
Biological Process	GO:0015942	formate metabolic process
Biological Process	GO:0035999	tetrahydrofolate interconversion

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.