Search Results

Overview

Uniprot IDA0A5G2R0S1
Protein NameLeucine--tRNA ligase, cytoplasmic
Gene NameLARS1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
1011 FASEAEDKVREDCCP
1020 REDCCPGKPLNVFRT
1097 RRVPVLGKEHTEKTP
133 KTEDIILKDKAKGKK
266 EYTLIKLKVLEPYPC
274 VLEPYPCKLSGLKGK
536 DYGEEKWKEQTCQCL
544 EQTCQCLKNLETFCE
929 HFETSSGKLPDNKVI
934 SGKLPDNKVIASELG
947 LGNLPELKKYMKKVM
966 MIKENLEKTGPRVLD
99 PIKACADKLKREIEL

Function

Aminoacyl-tRNA synthetase that catalyzes the specific attachment of leucine to its cognate tRNA (tRNA(Leu)). It performs tRNA aminoacylation in a two-step reaction: Leu is initially activated by ATP to form a leucyl-adenylate (Leu-AMP) intermediate; then the leucyl moiety is transferred to the acceptor 3' end of the tRNA to yield leucyl-tRNA. To improve the fidelity of catalytic reactions, it is also able to hydrolyze misactivated aminoacyl-adenylate intermediates (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing)

Protein Sequence

10 KGTTKVDFLK 20 KIEKEIQQKW 30 DTEKVFEVNA 40 SDLEKQTSKD 50 KYFVTFPYPY 60 MNGRLHLGHT 70 FSLSKCEFAV 80 GYQRLKGKTC 90 LYPFGLHCTG 100 MPIKACADKL 110 KREIELYGCP 120 PDFPDEEEEE 130 EEINAKTEDI 140 ILKDKAKGKK 150 SKASAKTGSS 160 KYQWDIMKSL 170 GLSDEEIVKF 180 SEAEHWLEYF 190 PPLAIQDLKR 200 MGLKVDWRRS 210 FITTDVNPYY 220 DSFVRWQFLT 230 LRERNKIKFG 240 KRYTIYSPKD 250 GQPCMDHDRQ 260 TGEGVGPQEY 270 TLIKLKVLEP 280 YPCKLSGLKG 290 KNVFLVAATL 300 RPETMFGQTN 310 CWVHPEIKYI 320 GFETVNGDIF 330 ICTPRAARNM 340 SYQGFTKDNG 350 VVPVVKELMG 360 EEILGASLSA 370 PLTSYKVIYV 380 LPMLTIKEDK 390 GTGVVTSVPS 400 DSPDDFAAFR 410 DLKKKQALRA 420 KYGIRDDMVL 430 PFEPVPIIEI 440 PGFGKLSAVT 450 LCDELKVQSQ 460 NDREKLAEAK 470 EKLYLKGFYD 480 GIMLVDGFEG 490 QKVQDVKKSI 500 RNRMIDTGDA 510 YAYMEPEKQV 520 MSRSSDECVV 530 ALCDQWYLDY 540 GEEKWKEQTC 550 QCLKNLETFC 560 EETRRNFEAT 570 LDWLQEHACS 580 RTYGLGTRLP 590 WDEQWLIESL 600 SDSTIYMAFY 610 TAAHLLQGGD 620 IRGQAESPLG 630 IRPQQMTKEV 640 WDYIFFKEAP 650 FPKTQIPKEK 660 LDQLKQEFEF 670 WYPVDLRVSG 680 KDLIPNHLSY 690 FLYNHVAMWP 700 EESDKWPVAV 710 RANGHLLLNS 720 EKMSKSTGNF 730 LTLTEALDKY 740 SADGMRLALA 750 DAGDTVEDAN 760 FVEAMADAGV 770 LRLYTWVEWV 780 KEMVANWDSL 790 RSGPANTFND 800 RVFAAKDKYR 810 ELAIEGMHRD 820 LVFRFIEVQT 830 LLLAPFCPHL 840 CEHIWTLLGK 850 PDSIMNASWP 860 VAGPVDESLI 870 RSSQYLMEVA 880 HDLRLRLKNY 890 MTPAKGKKPD 900 KQPPQKPSHC 910 TIYVAKDYPS 920 WQHVTLSVLR 930 SHFETSSGKL 940 PDNKVIASEL 950 GNLPELKKYM 960 KKVMPFVAMI 970 KENLEKTGPR 980 VLDLQLEFDE 990 QAVLMENIVY 1000 LTNSLELERI 1010 EVKFASEAED 1020 KVREDCCPGK 1030 PLNVFRTEPG 1040 VLVSLVNPQP 1050 FSGHFSAKIE 1060 IRQGDNRDSV 1070 IRRLMKVDRG 1080 IKDLSKVKLM 1090 RFDDPLLGPR 1100 RVPVLGKEHT 1110 EKTPISEQAV 1120 FHVDLTSKKV 1130 HLTENGLRAD 1140 IGDTMIYLVH

Gene Ontology

Classification GO ID Description
Cellular Component GO:0017101 aminoacyl-tRNA synthetase multienzyme complex
Molecular Function GO:0002161 aminoacyl-tRNA deacylase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004823 leucine-tRNA ligase activity
Biological Process GO:0006429 leucyl-tRNA aminoacylation

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.