Overview
| Uniprot ID | A0A5G2R2C9 |
|---|
| Protein Name | Apoptosis-inducing factor 1, mitochondrial |
|---|
| Gene Name | AIFM1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 228 |
VAVLTGKKVVQLDVR |
Function
Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Binds to DNA in a sequence-independent manner. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import
Protein Sequence
10
MFRCGGLAAG
20
ALKQKLAPLV
30
RTVCVRGPRQ
40
RNRLPVVQCH
50
HLGSPSRSLA
60
STGASGKDGS
70
SLVYFLIVGA
80
TVTGAGVYYA
90
YRTIKDNKKR
100
YNERIAGLGL
110
TPEEKQKRAT
120
SSAPEGEPVP
130
QVRVPSHVPF
140
LLIGGGTAAF
150
AAARSIRARD
160
PGARVLIVSE
170
DPELPYMRPP
180
LSKELWFSDD
190
PNVTKTLRFR
200
QWNGKERSIY
210
FQPPSFYVSA
220
QDLPHIENGG
230
VAVLTGKKVV
240
QLDVRGNMAK
250
LNDGSQITYE
260
KCLIATGGTP
270
RSLSAIDRAG
280
AEVKSRTTLF
290
RKIGDFRTLE
300
KISREVKSIT
310
IIGGGFLGSE
320
LACALGRKAR
330
ALGTEVIQLF
340
PEKGNMGKIL
350
PEYLSNWTME
360
KVRREGVKVL
370
PNAIVQSVGV
380
SGGKLLIKLK
390
DGRKVETDHI
400
VAAVGLEPNV
410
ELAKTGGLEI
420
DSDFGGFRVN
430
AELQARSNIW
440
VAGDAACFYD
450
IKLGRRRVEH
460
HDHAVVSGRL
470
AGENMTGAAK
480
PYWHQSMFWS
490
DLGPDVGYEA
500
IGLVDSSLPT
510
VGVFAKATAQ
520
DNPKSATEQS
530
GTGIRSESET
540
ESEAPEITLP
550
VGSPAVPQAP
560
TQGEDYGKGV
570
IFYLRDKVVV
580
GIVLWNVFNR
590
MPIARKIIKD
600
GEQHEDLNEV
AKLFNIHED
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005743 |
mitochondrial inner membrane |
| Cellular Component |
GO:0005758 |
mitochondrial intermembrane space |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0016491 |
oxidoreductase activity |
| Molecular Function |
GO:0046983 |
protein dimerization activity |
| Biological Process |
GO:0006915 |
apoptotic process |
| Biological Process |
GO:0043068 |
positive regulation of programmed cell death |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.