Overview
| Uniprot ID | A0A5G2R2L4 |
|---|
| Protein Name | Core-binding factor subunit beta |
|---|
| Gene Name | CBFB |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 11 |
VVPDQRSKFENEEFF |
| 200 |
QLLAVTGKKTARP** |
| 28 |
LSRECEIKYTGFRDR |
| 94 |
DLEREAGKVYLKAPM |
Function
Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation
Protein Sequence
10
MPRVVPDQRS
20
KFENEEFFRK
30
LSRECEIKYT
40
GFRDRPHEER
50
QARFQNACRD
60
GRSEIAFVAT
70
GTNLSLQFFP
80
ASWQGEQRQT
90
PSREYVDLER
100
EAGKVYLKAP
110
MILNGVCVIW
120
KGWIDLQRLD
130
GMGCLEFDEE
140
RAQVLSSLPM
150
FRVPLKNVGK
160
DVKQHLQEDA
170
LAQQAFEEAR
180
RRTREFEDRD
190
RSHREEMEVR
200
VSQLLAVTGK
KTARP
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0016513 |
core-binding factor complex |
| Molecular Function |
GO:0043565 |
sequence-specific DNA binding |
| Molecular Function |
GO:0003713 |
transcription coactivator activity |
| Biological Process |
GO:0043371 |
negative regulation of CD4-positive, alpha-beta T cell differentiation |
| Biological Process |
GO:0000122 |
negative regulation of transcription by RNA polymerase II |
| Biological Process |
GO:0043378 |
positive regulation of CD8-positive, alpha-beta T cell differentiation |
| Biological Process |
GO:0000209 |
protein polyubiquitination |
| Biological Process |
GO:0006357 |
regulation of transcription by RNA polymerase II |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.