Overview
| Uniprot ID | A0A5G2R3H6 |
|---|
| Protein Name | Eukaryotic translation initiation factor 3 subunit J |
|---|
| Gene Name | EIF3J |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 169 |
FGKLLKDKITQYEKS |
| 213 |
LTVLCSEKQKQEKQS |
| 227 |
SKAKKKKKGVVPGGG |
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA
Protein Sequence
10
MAAAAAAAAA
20
GDSDSWDADT
30
FSVDDPVRKV
40
GGGGTAGGDR
50
WEGEDEDEDV
60
KDNWDDDDDD
70
EKKEEAEVKP
80
EVKISEKKKI
90
AEKIKEKERQ
100
QKKRQEEIKK
110
RLEEPEEPKV
120
LTPEEQLADK
130
LRLKKLQEES
140
DLELAKETFG
150
VNNTIYGIDA
160
MNPSSRDDFT
170
EFGKLLKDKI
180
TQYEKSLYYA
190
SFLEALVRDV
200
CISLEIDDLK
210
KITNSLTVLC
220
SEKQKQEKQS
230
KAKKKKKGVV
240
PGGGLKATMK
250
DDLADYGGYD
260
GGYVQDYEDF
M
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0016282 |
eukaryotic 43S preinitiation complex |
| Cellular Component |
GO:0033290 |
eukaryotic 48S preinitiation complex |
| Cellular Component |
GO:0005852 |
eukaryotic translation initiation factor 3 complex |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0003743 |
translation initiation factor activity |
| Biological Process |
GO:0001732 |
formation of cytoplasmic translation initiation complex |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.