Overview
| Uniprot ID | A0A5G2R686 |
|---|
| Protein Name | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 |
|---|
| Gene Name | RPN1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 415 |
VSTLNSGKKSLELEH |
| 416 |
STLNSGKKSLELEHK |
| 423 |
KSLELEHKALTSEVA |
| 437 |
ALLQSRLKTEGSDLC |
| 458 |
QKLDAQVKELVLKSA |
| 463 |
QVKELVLKSAVEAER |
| 478 |
LVAGKLKKDTYIENE |
| 486 |
DTYIENEKLISGKRQ |
| 86 |
RNVESYTKLGNPTRS |
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity
Protein Sequence
10
MLKLSDQSGR
20
FFTVTLPVAL
30
DPGAKISVTV
40
ETVYTHVLQP
50
YPTQITQSEK
60
QFVVFEGNHY
70
FYSPYPTKSQ
80
SMRVKLASRN
90
VESYTKLGNP
100
TRSEDLLDYG
110
PFRDVPPYSQ
120
DTFKVHSENN
130
SPFLTITSMT
140
RVIEVSHWGN
150
IAVEENVDLK
160
HTGAVLKGPF
170
SRYDYQRQPD
180
SGISSIRSFK
190
TILPAAAQDV
200
YYRDEIGNVS
210
TSHLLILDDS
220
VEMEIRPRFP
230
LFGGWKTHYI
240
VGYNLPSYEY
250
LYNLGDQYAL
260
KMRLVDHVFD
270
EQVIDSLTVK
280
IILPEGAKNI
290
QVDSPYEISR
300
APDELHYTYL
310
DTFGRPVIVA
320
HKKNLVEQHI
330
QDIVVHYTFN
340
KVLMLQEPLL
350
VVAAFYILFF
360
TVIVYVRLDF
370
SITKDPAAEA
380
RMKVACITEQ
390
VLTLVNKRIG
400
LYRHFDETIN
410
RYKQSRDVST
420
LNSGKKSLEL
430
EHKALTSEVA
440
LLQSRLKTEG
450
SDLCDKVSEM
460
QKLDAQVKEL
470
VLKSAVEAER
480
LVAGKLKKDT
490
YIENEKLISG
500
KRQELVTKID
HILDAL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0008250 |
oligosaccharyltransferase complex |
| Biological Process |
GO:0009101 |
glycoprotein biosynthetic process |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.