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Overview

Uniprot IDA0A5G2R6F2
Protein NameLeucine--tRNA ligase, cytoplasmic
Gene NameLARS1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
1001 MIKENLEKTGPRVLD
103 PIKACADKLKREIEL
1046 FASEAEDKVREDCCP
1055 REDCCPGKPLNVFRT
1132 RRVPVLGKEHTEKTP
137 KTEDIILKDKAKGKK
270 EYTLIKLKVLEPYPC
278 VLEPYPCKLSGLKGK
540 DYGEEKWKEQTCQCL
548 EQTCQCLKNLETFCE
964 HFETSSGKLPDNKVI
969 SGKLPDNKVIASELG
982 LGNLPELKKYMKKVM

Function

Aminoacyl-tRNA synthetase that catalyzes the specific attachment of leucine to its cognate tRNA (tRNA(Leu)). It performs tRNA aminoacylation in a two-step reaction: Leu is initially activated by ATP to form a leucyl-adenylate (Leu-AMP) intermediate; then the leucyl moiety is transferred to the acceptor 3' end of the tRNA to yield leucyl-tRNA. To improve the fidelity of catalytic reactions, it is also able to hydrolyze misactivated aminoacyl-adenylate intermediates (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing)

Protein Sequence

10 MAERKGTTKV 20 DFLKKIEKEI 30 QQKWDTEKVF 40 EVNASDLEKQ 50 TSKDKYFVTF 60 PYPYMNGRLH 70 LGHTFSLSKC 80 EFAVGYQRLK 90 GKTCLYPFGL 100 HCTGMPIKAC 110 ADKLKREIEL 120 YGCPPDFPDE 130 EEEEEEINAK 140 TEDIILKDKA 150 KGKKSKASAK 160 TGSSKYQWDI 170 MKSLGLSDEE 180 IVKFSEAEHW 190 LEYFPPLAIQ 200 DLKRMGLKVD 210 WRRSFITTDV 220 NPYYDSFVRW 230 QFLTLRERNK 240 IKFGKRYTIY 250 SPKDGQPCMD 260 HDRQTGEGVG 270 PQEYTLIKLK 280 VLEPYPCKLS 290 GLKGKNVFLV 300 AATLRPETMF 310 GQTNCWVHPE 320 IKYIGFETVN 330 GDIFICTPRA 340 ARNMSYQGFT 350 KDNGVVPVVK 360 ELMGEEILGA 370 SLSAPLTSYK 380 VIYVLPMLTI 390 KEDKGTGVVT 400 SVPSDSPDDF 410 AAFRDLKKKQ 420 ALRAKYGIRD 430 DMVLPFEPVP 440 IIEIPGFGKL 450 SAVTLCDELK 460 VQSQNDREKL 470 AEAKEKLYLK 480 GFYDGIMLVD 490 GFEGQKVQDV 500 KKSIRNRMID 510 TGDAYAYMEP 520 EKQVMSRSSD 530 ECVVALCDQW 540 YLDYGEEKWK 550 EQTCQCLKNL 560 ETFCEETRRN 570 FEATLDWLQE 580 HACSRTYGLG 590 TRLPWDEQWL 600 IESLSDSTIY 610 MAFYTAAHLL 620 QGGDIRGQAE 630 SPLGIRPQQM 640 TKEVWDYIFF 650 KEAPFPKTQI 660 PKEKLDQLKQ 670 EFEFWYPVDL 680 RVSGKDLIPN 690 HLSYFLYNHV 700 AMWPEESDKW 710 PVAVRANGHL 720 LLNSEKMSKS 730 TGNFLTLTEA 740 LDKYSADGMR 750 LALADAGDTV 760 EDANFVEAMA 770 DAGVLRLYTW 780 VEWVKEMVAN 790 WDSLRSGPAN 800 TFNDRVFASE 810 MNAGIIKTDQ 820 NYKMMFKEAL 830 KTGFFEFQAA 840 KDKYRELAIE 850 GMHRDLVFRF 860 IEVQTLLLAP 870 FCPHLCEHIW 880 TLLGKPDSIM 890 NASWPVAGPV 900 DESLIRSSQY 910 LMEVAHDLRL 920 RLKNYMTPAK 930 GKKPDKQPPQ 940 KPSHCTIYVA 950 KDYPSWQHVT 960 LSVLRSHFET 970 SSGKLPDNKV 980 IASELGNLPE 990 LKKYMKKVMP 1000 FVAMIKENLE 1010 KTGPRVLDLQ 1020 LEFDEQAVLM 1030 ENIVYLTNSL 1040 ELERIEVKFA 1050 SEAEDKVRED 1060 CCPGKPLNVF 1070 RTEPGVLVSL 1080 VNPQPFSGHF 1090 SAKIEIRQGD 1100 NRDSVIRRLM 1110 KVDRGIKDLS 1120 KVKLMRFDDP 1130 LLGPRRVPVL 1140 GKEHTEKTPI 1150 SEQAVFHVDL 1160 TSKKVHLTEN 1170 GLRADIGDTM IYLVH

Gene Ontology

Classification GO ID Description
Cellular Component GO:0017101 aminoacyl-tRNA synthetase multienzyme complex
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0005764 lysosome
Molecular Function GO:0002161 aminoacyl-tRNA deacylase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004819 glutamine-tRNA ligase activity
Molecular Function GO:0005096 GTPase activator activity
Molecular Function GO:0004823 leucine-tRNA ligase activity
Biological Process GO:0071233 cellular response to L-leucine
Biological Process GO:1990253 cellular response to leucine starvation
Biological Process GO:0006425 glutaminyl-tRNA aminoacylation
Biological Process GO:0006429 leucyl-tRNA aminoacylation
Biological Process GO:1904263 positive regulation of TORC1 signaling

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.