Overview
| Uniprot ID | A0A5G2RAI6 |
|---|
| Protein Name | Phosphatidylinositol-binding clathrin assembly protein |
|---|
| Gene Name | PICALM |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 134 |
YSRYLNEKAVSYRQV |
| 147 |
QVAFDFTKVKRGADG |
| 231 |
DMKKNQCKEGLDIYK |
| 24 |
VTGSAVSKTVCKATT |
| 324 |
TKVDEREKQAALEEE |
Function
Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF)
Protein Sequence
10
MSGQSLTDRI
20
TAAQHSVTGS
30
AVSKTVCKAT
40
THEIMGPKKK
50
HLDYLIQCTN
60
EMNVNIPQLA
70
DSLFERTTNS
80
SWVVVFKSLI
90
TTHHLMVYGN
100
ERFIQYLASR
110
NTLFNLSNFL
120
DKSGLQGYDM
130
STFIRRYSRY
140
LNEKAVSYRQ
150
VAFDFTKVKR
160
GADGVMRTMN
170
TEKLLKTVPI
180
IQNQMDALLD
190
FNVNSNELTN
200
GVINAAFMLL
210
FKDAIRLFAA
220
YNEGIINLLE
230
KYFDMKKNQC
240
KEGLDIYKKF
250
LTRMTRISEF
260
LKVAEQVGID
270
RGDIPDLSQA
280
PSSLLDALEQ
290
HLASLEGKKI
300
KDSTAASRAT
310
TLSNAVSSLA
320
STGLSLTKVD
330
EREKQAALEE
340
EQARLKALKE
350
QRLKELAKKP
360
HTSLTTAASP
370
VSTSAGGIMT
380
APAIDIFSTP
390
SSSNSTSKLP
400
NDLLDLQQPT
410
FHPSVLPMSS
420
ASQVASTWGD
430
PFSATVDAVD
440
DAIPSLNPFL
450
TKSSGDVHLP
460
ISSDVSTFTT
470
RTPTHEMFVD
480
SFCGTQAYPN
490
TTFMCHEPST
500
VAGLFGGFTP
510
SPVAQPHPSA
520
GLNVDFESVF
530
GNKSTNVIVD
540
SGGGLLKPTV
550
ASQNQSLPVA
560
KLPPNKLVSD
570
DLDSSLANLV
580
GNLGIGNGTT
590
KNDVNWSQPG
600
EKKLTGGSNW
610
QPKVAPTTAW
620
NAATMAPPVM
630
AYPATTPTGM
640
IGYGIPPQMG
650
SVPVMTQPTL
660
IYSQPVMRPP
670
NPFGPVSGAQ
680
LSAASSPSSH
690
SPHRASGKDP
700
FAELSLEDFL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0009986 |
cell surface |
| Cellular Component |
GO:0030132 |
clathrin coat of coated pit |
| Cellular Component |
GO:0030136 |
clathrin-coated vesicle |
| Cellular Component |
GO:0005769 |
early endosome |
| Cellular Component |
GO:0070381 |
endosome to plasma membrane transport vesicle |
| Cellular Component |
GO:0005794 |
Golgi apparatus |
| Cellular Component |
GO:0097418 |
neurofibrillary tangle |
| Cellular Component |
GO:0043025 |
neuronal cell body |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Molecular Function |
GO:0005545 |
1-phosphatidylinositol binding |
| Molecular Function |
GO:0032050 |
clathrin heavy chain binding |
| Molecular Function |
GO:0050750 |
low-density lipoprotein particle receptor binding |
| Molecular Function |
GO:0031267 |
small GTPase binding |
| Molecular Function |
GO:0000149 |
SNARE binding |
| Molecular Function |
GO:0048156 |
tau protein binding |
| Biological Process |
GO:0150093 |
amyloid-beta clearance by transcytosis |
| Biological Process |
GO:0007409 |
axonogenesis |
| Biological Process |
GO:0048268 |
clathrin coat assembly |
| Biological Process |
GO:0072583 |
clathrin-dependent endocytosis |
| Biological Process |
GO:0048813 |
dendrite morphogenesis |
| Biological Process |
GO:0016197 |
endosomal transport |
| Biological Process |
GO:0030097 |
hemopoiesis |
| Biological Process |
GO:0006879 |
intracellular iron ion homeostasis |
| Biological Process |
GO:0007611 |
learning or memory |
| Biological Process |
GO:0097753 |
membrane bending |
| Biological Process |
GO:0060586 |
multicellular organismal-level iron ion homeostasis |
| Biological Process |
GO:0010629 |
negative regulation of gene expression |
| Biological Process |
GO:2000009 |
negative regulation of protein localization to cell surface |
| Biological Process |
GO:1903077 |
negative regulation of protein localization to plasma membrane |
| Biological Process |
GO:0048261 |
negative regulation of receptor-mediated endocytosis |
| Biological Process |
GO:1902004 |
positive regulation of amyloid-beta formation |
| Biological Process |
GO:0045893 |
positive regulation of DNA-templated transcription |
| Biological Process |
GO:0046579 |
positive regulation of Ras protein signal transduction |
| Biological Process |
GO:0031623 |
receptor internalization |
| Biological Process |
GO:0097494 |
regulation of vesicle size |
| Biological Process |
GO:0016188 |
synaptic vesicle maturation |
| Biological Process |
GO:0006900 |
vesicle budding from membrane |
| Biological Process |
GO:0035459 |
vesicle cargo loading |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.