Overview
| Uniprot ID | A0A5G2RB73 |
|---|
| Protein Name | Vesicle-associated membrane protein-associated protein A |
|---|
| Gene Name | VAPA |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 125 |
PDELMDSKLRCVFEM |
| 179 |
PRGLSVFKQEKQKND |
| 199 |
AAPLNAAKQDGPVPK |
| 219 |
LSDADTRKLVEECKR |
| 225 |
RKLVEECKRLQGEMV |
| 233 |
RLQGEMVKLSEENRH |
| 250 |
DEGLRLRKVAHSEKP |
| 256 |
RKVAHSEKPGSTSAA |
| 38 |
DVVTTNLKLRNPSDR |
| 52 |
RKVCFKVKTTAPRRY |
Function
Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44. STARD3-VAPA interaction enables cholesterol transfer from the ER to endosomes. Via interaction with WDR44 participates in neosynthesized protein export. In addition, recruited to the plasma membrane through OSBPL3 binding. The OSBPL3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. With OSBPL3, may regulate ER morphology. May play a role in vesicle trafficking
Protein Sequence
10
MASASGAMAK
20
HEQILVLDPP
30
TDLKFKGPFT
40
DVVTTNLKLR
50
NPSDRKVCFK
60
VKTTAPRRYC
70
VRPNSGIIDP
80
GLTVTVSVML
90
QPFDYDPNEK
100
SKHKFMVQTI
110
FAPPNITDME
120
AVWKEAKPDE
130
LMDSKLRCVF
140
EMPNENDKLG
150
TAPPGTALAA
160
TPLSTTTSTV
170
AAPASYSTKS
180
EPRGLSVFKQ
190
EKQKNDMEPS
200
KAAPLNAAKQ
210
DGPVPKPHSV
220
SLSDADTRKL
230
VEECKRLQGE
240
MVKLSEENRH
250
LRDEGLRLRK
260
VAHSEKPGST
270
SAASFRDNVT
280
SPLPSLLVKK
290
ICLPTISLVV
300
WPTVTILCVY
SVM
Gene Ontology
| Classification |
GO ID |
Description |
| Biological Process |
GO:0015914 |
phospholipid transport |
| Cellular Component |
GO:0005923 |
bicellular tight junction |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0000139 |
Golgi membrane |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0031982 |
vesicle |
| Molecular Function |
GO:0033149 |
FFAT motif binding |
| Molecular Function |
GO:0008017 |
microtubule binding |
| Molecular Function |
GO:0046982 |
protein heterodimerization activity |
| Molecular Function |
GO:0042803 |
protein homodimerization activity |
| Molecular Function |
GO:0043495 |
protein-membrane adaptor activity |
| Biological Process |
GO:0035627 |
ceramide transport |
| Biological Process |
GO:0042632 |
cholesterol homeostasis |
| Biological Process |
GO:0030301 |
cholesterol transport |
| Biological Process |
GO:0090114 |
COPII-coated vesicle budding |
| Biological Process |
GO:0061817 |
endoplasmic reticulum-plasma membrane tethering |
| Biological Process |
GO:0044829 |
host-mediated activation of viral genome replication |
| Biological Process |
GO:0044828 |
host-mediated suppression of viral genome replication |
| Biological Process |
GO:0031175 |
neuron projection development |
| Biological Process |
GO:0070972 |
protein localization to endoplasmic reticulum |
| Biological Process |
GO:0006686 |
sphingomyelin biosynthetic process |
| Biological Process |
GO:0019076 |
viral release from host cell |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.