Overview
| Uniprot ID | A0A5G2RF15 |
|---|
| Protein Name | DNA-directed RNA polymerase subunit |
|---|
| Gene Name | POLR2G |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 132 |
VLYPVKYKAIVFRPF |
| 205 |
QDDEIRLKIVGTRVD |
Function
Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. POLR2G/RPB7 is part of a subcomplex with POLR2D/RPB4 that binds to a pocket formed by POLR2A/RPB1, POLR2B/RPB2 and POLR2F/RPABC2 at the base of the clamp element. The POLR2D/RPB4-POLR2G/RPB7 subcomplex seems to lock the clamp via POLR2G/RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The POLR2D/RPB4-POLR2G/RPB7 subcomplex binds single-stranded DNA and RNA
Protein Sequence
10
RACADRKGRT
20
ELKAFKCLKR
30
VDWTKGEELR
40
GFPRPRKRGP
50
IKSADETCSR
60
PPLWGRPGSG
70
IGEISLEHEI
80
LLHPRYFGPN
90
LLNTVKQKLF
100
TEVEGTCTGK
110
YGFVIAVTTI
120
DNIGAGVIQP
130
GRGFVLYPVK
140
YKAIVFRPFK
150
GEVVDAVVTQ
160
VNKVGLFTEI
170
GPMSCFISRH
180
SIPSEMEFDP
190
NSNPPCYKTM
200
DEDIVIQQDD
210
EIRLKIVGTR
220
VDKNDIFAIG
230
SLMDDYLGLV
S
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000932 |
P-body |
| Cellular Component |
GO:0005665 |
RNA polymerase II, core complex |
| Molecular Function |
GO:0003697 |
single-stranded DNA binding |
| Molecular Function |
GO:0003727 |
single-stranded RNA binding |
| Molecular Function |
GO:0031369 |
translation initiation factor binding |
| Biological Process |
GO:0000956 |
nuclear-transcribed mRNA catabolic process |
| Biological Process |
GO:0060213 |
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening |
| Biological Process |
GO:0045948 |
positive regulation of translational initiation |
| Biological Process |
GO:0006368 |
transcription elongation by RNA polymerase II |
| Biological Process |
GO:0006367 |
transcription initiation at RNA polymerase II promoter |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.