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Overview

Uniprot IDA0A5K1U589
Protein NameUbiquitin-like modifier-activating enzyme 1
Gene NameUBA1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
1003 KQFLDYFKTEHKLEI
148 LREEDIGKNRAEVSQ
208 FCHSRGIKLVVADTR
319 GGIVSQVKVPKKISF
466 KEALTEDKCLPRQNR
549 FRPWDVTKLKSDTAA
551 PWDVTKLKSDTAAAA
616 RRCVYYRKPLLESGT
694 NQYLTDPKFVERTLR
853 DSRLEELKATLPSPE
907 PADRHKSKLIAGKII

Function

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites

Protein Sequence

10 MLGWGGRRMA 20 WGSAAGIASQ 30 TTWMSSSPLS 40 KKRRVSGPDP 50 KPGSNCSPAH 60 SVLSEVPSVP 70 TNGMAKNGSE 80 ADIDEGLYSR 90 QLYVLGHEAM 100 KRLQTSSVLV 110 SGLRGLGVEI 120 AKNIILGGVK 130 AVTLHDQGTA 140 QWADLSSQFY 150 LREEDIGKNR 160 AEVSQPRLAE 170 LNSYVPVSAY 180 TGPLVEDFLS 190 GFQVVVLTNT 200 PLEDQLRVGE 210 FCHSRGIKLV 220 VADTRGLFGQ 230 LFCDFGEEMI 240 LTDSNGEQPL 250 SAMVSMVTKD 260 NPGVVTCLDE 270 ARHGFESGDF 280 VSFSEVQGMI 290 ELNGSQPMEI 300 KVLGPYTFSI 310 CDTSGFSDYI 320 RGGIVSQVKV 330 PKKISFKSLL 340 ASLAEPDFVM 350 TDFAKYSRPA 360 QLHIGFQALH 370 QFCAQHGRPP 380 RPRNEEDATE 390 LVTLARAVNA 400 RALPAVQQDS 410 LDEDLIRKLA 420 YVAAGDLAPI 430 NAFIGGLAAQ 440 EVMKACSGKF 450 MPIMQWLYFD 460 ALECLPEDKE 470 ALTEDKCLPR 480 QNRFDGQVAV 490 FGSDLQEKLG 500 KQKYFLVGAG 510 AIGCELLKNF 520 AMIGLGCGEG 530 GEIVVTDMDT 540 IEKSNLNRQF 550 LFRPWDVTKL 560 KSDTAAAAVR 570 QMNPHIRVTS 580 HQNRVGPDTE 590 RIYDDDFFQN 600 LDGVANALDN 610 VDARMYMDRR 620 CVYYRKPLLE 630 SGTLGTKGNV 640 QVVIPFLTES 650 YSSSQDPPEK 660 SIPICTLKNF 670 PNAIEHTLQW 680 ARDEFEGLFK 690 QPAENVNQYL 700 TDPKFVERTL 710 RLAGTQPLEV 720 LEAVQRSLVL 730 QRPQTWADCV 740 TWACHHWHTQ 750 YSNNIRQLLH 760 NFPPDQLTSS 770 GAPFWSGPKR 780 CPHPLTFDVN 790 NPLHLDYVMA 800 AANLFAQTYG 810 LTGSQDRAAV 820 ATLLQSVHVP 830 EFTPKSGVKI 840 HVSDQELQSA 850 NASVDDSRLE 860 ELKATLPSPE 870 KLSGFKMYPI 880 DFEKDDDSNF 890 HMDFIVAASN 900 LRAENYDIPP 910 ADRHKSKLIA 920 GKIIPAIATT 930 TAAVVGLVCL 940 ELYKVVQGHR 950 QLDSYKNGFL 960 NLALPFFGFS 970 EPLAAPRHQY 980 YNQEWTLWDR 990 FEVQGLQPNG 1000 EEMTLKQFLD 1010 YFKTEHKLEI 1020 TMLSQGVSML 1030 YSFFMPAAKL 1040 KERLDQPMTE 1050 IVSRVSKRKL 1060 GRHVRALVLE 1070 LCCNDESGED 1080 VEVPYVRYTI R

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0030057 desmosome
Cellular Component GO:0010008 endosome membrane
Cellular Component GO:0000792 heterochromatin
Cellular Component GO:0005765 lysosomal membrane
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0030867 rough endoplasmic reticulum membrane
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004839 ubiquitin activating enzyme activity
Biological Process GO:0006974 DNA damage response
Biological Process GO:0016567 protein ubiquitination
Biological Process GO:0006511 ubiquitin-dependent protein catabolic process

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.