Overview
| Uniprot ID | A0A8D0NHD1 |
|---|
| Protein Name | Pseudouridylate synthase 1 homolog |
|---|
| Gene Name | PUS1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 153 |
QRCARTDKGVSAAGQ |
| 199 |
VTGGFNSKNKCDART |
Function
Pseudouridylate synthase that catalyzes pseudouridylation of tRNAs and mRNAs. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'. Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions. Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing. Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 mRNA
Protein Sequence
10
MGLPNLWAAA
20
WALRRVCGPW
30
TPRLGPRLLC
40
LPPMAGNGEA
50
PVPVGTGQEQ
60
DKKARSGWQG
70
AARIWEETEQ
80
QAKKLKSSED
90
GEQRRKLPKR
100
KIVLLMAYSG
110
KGYHGMQRNV
120
GSSQFKTIED
130
DLVSALVRSG
140
CIPENHGEDM
150
RKMSFQRCAR
160
TDKGVSAAGQ
170
VVSLKVWLID
180
DILEKINSHL
190
PSHIRILGLK
200
RVTGGFNSKN
210
KCDARTYFYM
220
LPTFAFAHKD
230
HDVQDETYRL
240
STETLQRVNR
250
LLACYKGTHN
260
FHNFTSQKGP
270
REPSARRYIL
280
DMFCEEPFVR
290
EGMEFAVIKV
300
KGQSFMTHQI
310
RKMVGLVVAI
320
IKGYAPESVL
330
ERSWGEAKVD
340
VPKAPGLGLV
350
LERVHFEKYN
360
QRFGHDGVHE
370
PLDWAREEAE
380
VMAFKEQHIY
390
PTIISTERHE
400
KSMAQWLSTL
410
PMHNFSATAQ
420
EAAGLGSKDP
430
NPLEGSEGDG
DSD
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0005730 |
nucleolus |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005667 |
transcription regulator complex |
| Molecular Function |
GO:0003682 |
chromatin binding |
| Molecular Function |
GO:0160153 |
mitochondrial tRNA pseudouridine(27/28) synthase activity |
| Molecular Function |
GO:0009982 |
pseudouridine synthase activity |
| Molecular Function |
GO:0002153 |
steroid receptor RNA activator RNA binding |
| Molecular Function |
GO:0003713 |
transcription coactivator activity |
| Molecular Function |
GO:0000049 |
tRNA binding |
| Molecular Function |
GO:0160147 |
tRNA pseudouridine(38-40) synthase activity |
| Biological Process |
GO:0006397 |
mRNA processing |
| Biological Process |
GO:1990481 |
mRNA pseudouridine synthesis |
| Biological Process |
GO:0031119 |
tRNA pseudouridine synthesis |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.