Overview
| Uniprot ID | A0A8D0PAY4 |
|---|
| Protein Name | Eukaryotic translation initiation factor 3 subunit H |
|---|
| Gene Name | EIF3H |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 178 |
PKLMEVCKEKDFSPE |
| 180 |
LMEVCKEKDFSPEAL |
| 188 |
DFSPEALKKGNITFE |
| 227 |
KKSAVADKHELLSLA |
| 306 |
EDLSKLFKPHQPPAR |
| 5 |
***MASRKEGTGSAA |
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression
Protein Sequence
10
MASRKEGTGS
20
AATSSSSTTG
30
AAGKGKGKGG
40
SGDSAVKQVQ
50
IDGLVVLKII
60
KHYQEEGQGT
70
EVVQGVLLGL
80
VVEDRLEITN
90
CFPFPQHTED
100
DADFDEVQYQ
110
MEMMRSLRHV
120
NIDHLHVGWY
130
QSTYYGSFVT
140
RALLDSQFSY
150
QHAIEESVVL
160
IYDPIKTAQG
170
SLSLKAYRLT
180
PKLMEVCKEK
190
DFSPEALKKG
200
NITFEHMFEE
210
VPIVIKNSHL
220
INVLMWELEK
230
KSAVADKHEL
240
LSLASSNHLG
250
KNLQLLMDRV
260
DEMSQDIVKY
270
NTYLRNTSKQ
280
QQQKHQYQQR
290
RQQENMQRQS
300
RGEPPLPEED
310
LSKLFKPHQP
320
PARMDSLLIA
GIIS
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0016282 |
eukaryotic 43S preinitiation complex |
| Biological Process |
GO:0001732 |
formation of cytoplasmic translation initiation complex |
| Cellular Component |
GO:0033290 |
eukaryotic 48S preinitiation complex |
| Cellular Component |
GO:0005852 |
eukaryotic translation initiation factor 3 complex |
| Molecular Function |
GO:0008237 |
metallopeptidase activity |
| Molecular Function |
GO:0003743 |
translation initiation factor activity |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.