Overview
| Uniprot ID | A0A8D0YK35 |
|---|
| Protein Name | PAT complex subunit CCDC47 |
|---|
| Gene Name | CCDC47 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 273 |
LSEFCSDKPKSGAKY |
Function
Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes. The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions. Within the MPT complex, the PAT subcomplex sequesters any highly polar regions in the transmembrane domains away from the non-polar membrane environment until they can be buried in the interior of the fully assembled protein. Within the PAT subcomplex, CCDC47 occludes the lateral gate of the SEC61 complex. Involved in the regulation of calcium ion homeostasis in the ER. Required for proper protein degradation via the ERAD (ER-associated degradation) pathway. Has an essential role in the maintenance of ER organization during embryogenesis
Protein Sequence
10
MKALHAFCVV
20
LLIFGSFSEA
30
KFDDFEDEED
40
IVEYDDNDFA
50
EFEDVPEDSV
60
TESPQRVITT
70
EDDEDETTVE
80
LEGQDENQEG
90
DFEDADTQEG
100
DTESEPYDDE
110
EFEGYEDKPD
120
TSSSKSKDPI
130
TIVDVPAHLQ
140
NSWESYYLEI
150
LMVTGLLAYI
160
MNYIIGKNKN
170
SRLAQAWFNT
180
HRELLESNFT
190
LVGDDGTNKE
200
ATSTGKLNQE
210
NEHIYNLWCS
220
GRVCCEGMLI
230
QLRVSREFLI
240
QCQIKVTMND
250
EDMDTYVFAV
260
GTRKALVRLQ
270
KEMQDLSEFC
280
SDKPKSGAKY
290
GLPDSLAILS
300
EMGEVTEGMM
310
DTKMVHFLTH
320
YADKIESVHF
330
SDQFSGPKIM
340
QEEGQPLKLP
350
DTKRTLLFTF
360
NVPGSGNTYP
370
KDMEALLPLM
380
NMVIYSIDKA
390
KKFRLNREGK
400
QKADKNRARV
410
EENFLKLTHV
420
QRQEAAQSRR
430
EEKKRAEKER
440
IMNEEDPEKQ
450
RRLEEAALRR
460
EQKKLEKKQM
470
KMKQIKVKAM
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0030867 |
rough endoplasmic reticulum membrane |
| Molecular Function |
GO:0005509 |
calcium ion binding |
| Biological Process |
GO:0032469 |
endoplasmic reticulum calcium ion homeostasis |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.