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Overview

Uniprot IDA0A8D0YSZ7
Protein NamePyruvate kinase
Gene NamePKM
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
175 SRSVETLKEMIKSGM
179 ETLKEMIKSGMNVAR
202 EYHAETIKNVRAATE
228 VAVALDTKGPEIRTG
248 GTAEVELKKGATLKI
279 LDYKNICKVVDVGSK
320 GGFLGSKKGVNLPGA
360 VFASFIRKAADVHEV
374 VRKVLGEKGKNIKII
379 GEKGKNIKIISKIEN
383 KNIKIISKIENHEGV
506 EAAMFHRKLFEELVR
611 NLAMNVGKARGFFKK

Function

Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival

Protein Sequence

10 MLAWRHGAVG 20 SKLMLQQGTA 30 VDIRGEMALR 40 KILLQGLRLK 50 NKAPWTGHSM 60 CCHQWICPAS 70 TAGLSSAGQT 80 FLRSEQSLQV 90 GPRSHPEPTK 100 EHYITGSPSP 110 ENTSSSGPKT 120 SEAMPKPHSD 130 AGTAFIQTQQ 140 LHAAMADTFL 150 EHMCRLDIDS 160 PPITARNTGI 170 ICTIGPASRS 180 VETLKEMIKS 190 GMNVARLNFS 200 HGTHEYHAET 210 IKNVRAATES 220 FASDPILYRP 230 VAVALDTKGP 240 EIRTGLIKGS 250 GTAEVELKKG 260 ATLKITLDNA 270 YMEKCDENVL 280 WLDYKNICKV 290 VDVGSKVYVD 300 DGLISLLVKQ 310 KGPDFLVTEV 320 ENGGFLGSKK 330 GVNLPGAAVD 340 LPAVSEKDIQ 350 DLKFGVEQDV 360 DMVFASFIRK 370 AADVHEVRKV 380 LGEKGKNIKI 390 ISKIENHEGV 400 RRFDEILEAS 410 DGIMVARGDL 420 GIEIPAEKVF 430 LAQKMMIGRC 440 NRAGKPVICA 450 TQMLESMIKK 460 PRPTRAEGSD 470 VANAVLDGAD 480 CIMLSGETAK 490 GDYPLEAVRM 500 QHLIAREAEA 510 AMFHRKLFEE 520 LVRASSHSTD 530 LMEAMAMGSV 540 EASYKCLAAA 550 LIVLTESGRS 560 AHQVARYRPR 570 APIIAVTRNH 580 QTARQAHLYR 590 GIFPVVCKDP 600 VQEAWAEDVD 610 LRVNLAMNVG 620 KARGFFKKGD 630 VVIVLTGWRP 640 GSGFTNTMRV VPVP

Gene Ontology

Classification GO ID Description
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016301 kinase activity
Molecular Function GO:0000287 magnesium ion binding
Molecular Function GO:0030955 potassium ion binding
Molecular Function GO:0004743 pyruvate kinase activity

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.