Overview
| Uniprot ID | A0A8D1MYQ1 |
|---|
| Protein Name | Transcription initiation factor IIB |
|---|
| Gene Name | GTF2B |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 177 |
EGVPRTFKEICAVSR |
| 237 |
AATHIARKAVELDLV |
Function
General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element. Modulates transcription start site selection. Also exhibits autoacetyltransferase activity that contributes to the activated transcription
Protein Sequence
10
MSSNHLDALP
20
RVTCPNHPDA
30
ILVEDYRAGD
40
MICPECGLVV
50
GDRVIDVGSE
60
WRTFSNDKAT
70
KDPSRVGDSQ
80
NPLLSDGDLS
90
TMIGKGTGAA
100
SFDEFGNSKY
110
QNRRTMSSSD
120
RAMMNAFKEI
130
TTMADRINLP
140
RNIVDRTNNL
150
FKQVYEQKSL
160
KGRANDAIAS
170
ACLYIACRQE
180
GVPRTFKEIC
190
AVSRISKKEI
200
GRCFKLILKA
210
LETSVDLITT
220
GDFMSRFCSN
230
LCLPKQVQMA
240
ATHIARKAVE
250
LDLVPGRSPI
260
SVAAAAIYMA
270
SQASAEKRTQ
280
KEIGDIAGVA
290
DVTIRQSYRL
300
IYPRAPDLFP
310
TDFKFDTPVD
KLPQL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005694 |
chromosome |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:1990841 |
promoter-specific chromatin binding |
| Molecular Function |
GO:0061733 |
protein-lysine-acetyltransferase activity |
| Molecular Function |
GO:0017025 |
TBP-class protein binding |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0070897 |
transcription preinitiation complex assembly |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.