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Overview

Uniprot IDA0A8D1SY88
Protein NameSerine palmitoyltransferase 1
Gene NameSPTLC1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
115 LLDNPRVKAAALASL
123 AAALASLKKYGVGTC
194 AACFAIQKGLQASRS
372 EKCKRIHKALQGISG

Function

Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases. The SPT complex is also composed of SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer with SPTLC2 or SPTLC3 forms the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Required for adipocyte cell viability and metabolic homeostasis

Protein Sequence

10 GIKAETSWFL 20 VGFVSTAPAY 30 HLILEGILIL 40 WIIRLLFSKT 50 YKLQERSDLT 60 VKEKEELIEE 70 WQPEPLVPPV 80 SKDHPALNYN 90 IVSGPPSHNI 100 VVNGKECINF 110 ASFNFLGLLD 120 NPRVKAAALA 130 SLKKYGVGTC 140 GPRGFYGTFD 150 VHLDLEDRLA 160 KFMKTEEAII 170 YSYGFATIAS 180 AIPAYSKRGD 190 IVFVDKAACF 200 AIQKGLQASR 210 SDIKLFEHND 220 MEDLERLLKE 230 QEIEDQKNPR 240 KARVTRRFIV 250 VEGLYMNTGT 260 ICPLPELVKL 270 KYKYKARIFL 280 EESLSFGVLG 290 EHGRGVTEHF 300 GINIDDIDLI 310 SANMENSLAS 320 IGGFCCGRSF 330 VIDHQRLSGQ 340 GYCFSASLPP 350 LLAAAAIEAL 360 NIMEENPGIF 370 AVLKEKCKRI 380 HKALQGISGL 390 KVAGESISPA 400 FHLQLEESTG 410 SRDKDVKLLQ 420 EIVTGCMDRG 430 IALTQARYLE 440 KEEKNLPPPS 450 IRVVVTVEQT 460 EEELDKAAST 470 INEVAQIVLL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0016020 membrane
Molecular Function GO:0030170 pyridoxal phosphate binding
Molecular Function GO:0004758 serine C-palmitoyltransferase activity
Biological Process GO:0043604 amide biosynthetic process
Biological Process GO:0030148 sphingolipid biosynthetic process

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.