Search Results

Overview

Uniprot ID	A0A8D1TZC9
Protein Name	Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
Gene Name	PCK2
Organism	Sus scrofa

Kla Sites from experimental identification

Position	Flanking peptide
102	DVARVESKTVIVTPS
255	GGNSLLGKKCFALRI

Function

Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Can play an active role in glyceroneogenesis and gluconeogenesis. Also acts as a serine/threonine-protein kinase: phosphorylates and activates ACSL4, thereby promoting ferroptosis

Protein Sequence

10 MATLLSWHGL 20 SSWGWSSRRS 30 IQALRVLNGD 40 LGQLPAGVRD 50 FVERSARLCQ 60 PEGIHICDGT 70 EAENTATLAL 80 LEQQGLIRKL 90 PKYNNCWLAR 100 TDPKDVARVE 110 SKTVIVTPSQ 120 RDTVPLPAGG 130 ARGQLGNWMS 140 PAEFQQAVDE 150 RFPGCMQGRT 160 MYVLPFSMGP 170 VGSPLSRIGV 180 QLTDSAYVVA 190 SMRIMTRLGK 200 PVLQALGDGD 210 FVKCLHSVGQ 220 PLTGQGEPVS 230 QWPCNPEKTL 240 IGHVPDQREI 250 VSFGSGYGGN 260 SLLGKKCFAL 270 RIASRLARDE 280 GWLAEHMLIL 290 GITNPAGKKR 300 YVAAAFPSAC 310 GKTNLAMMRP 320 ALPGWKVECV 330 GDDIAWMRFD 340 SDGQLRAINP 350 ENGFFGVAPG 360 TSATTNPNAM 370 ATIQSNTLFT 380 NVAETSDGGV 390 YWEGIDQPLP 400 PGVTVTSWLG 410 KPWKPGDKEP 420 CAHPNSRFCA 430 PARQCPIMDP 440 AWEAPEGVPI 450 DAIIFGGRRP 460 KGVPLVYEAF 470 SWRHGVFVGS 480 AMRSESTAAA 490 EHKGKVIMHD 500 PFAMRPFFGY 510 NFGRYLEHWL 520 SMEGRKGARL 530 PRIFHVNWFR 540 RDEAGHFLWP 550 GFGENARVLD 560 WICRRLEQED 570 SARETPIGLV 580 PKEGALDLRG 590 LGAIDTTQLF 600 SIPKDFWEQE 610 VRDIRSYLTE 620 QVNQDLPKEV 630 LAELEALEGR VRRM

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005739	mitochondrion
Molecular Function	GO:0005525	GTP binding
Molecular Function	GO:0016301	kinase activity
Molecular Function	GO:0046872	metal ion binding
Molecular Function	GO:0004613	phosphoenolpyruvate carboxykinase (GTP) activity
Biological Process	GO:0006094	gluconeogenesis

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.