Overview
| Uniprot ID | A0A8D1WF95 |
|---|
| Protein Name | Lipid droplet-regulating VLDL assembly factor AUP1 |
|---|
| Gene Name | AUP1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 97 |
GELVESLKRFCASTR |
Function
Plays a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. Plays a role in lipid droplet formation. Induces lipid droplet clustering. Recruits ubiquitin-conjugating enzyme UBE2G2 to lipid droplets which facilitates its interaction with ubiquitin ligases AMFR/gp78 and RNF139/TRC8, leading to sterol-induced ubiquitination of HMGCR and its subsequent proteasomal degradation. Also required for the degradation of INSIG1, SREBF1 and SREBF2. Plays a role in regulating assembly and secretion of very low density lipoprotein particles and stability of apolipoprotein APOB
Protein Sequence
10
MEPSPAPGPE
20
RLFDSHRFVV
30
RTMCAVLGLV
40
ARQEDSGLRD
50
HRVRVLISNH
60
VTPFDHNIVN
70
LLTSCSTPLL
80
NSPPSFVCWS
90
RGFMEMDGRG
100
ELVESLKRFC
110
ASTRLPPTPL
120
LLFPEEEATN
130
GREGLLRFSS
140
WPFSIQDVVQ
150
PLTLRVQRPL
160
VSVTVSDASW
170
VSELLWSLFV
180
PFTVYQVRWL
190
RPVHRQLGEG
200
NEEFALRVQQ
210
LLAKELGQTG
220
TRLTPADKAE
230
HMKRQRHPRL
240
RPQSAQSSFP
250
PSPGPSEAQL
260
ATLAQRVKEV
270
LPHVPLGVIQ
280
RDLARTGCVD
290
LTITNLLEGA
300
VAFMPEDITE
310
GTQPLPTAST
320
PKFPSSGPAT
330
PQPTALTFAK
340
SSWARQESLQ
350
ERKQALYEYA
360
RRRFTEKQAQ
EAD
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0005811 |
lipid droplet |
| Molecular Function |
GO:0043130 |
ubiquitin binding |
| Biological Process |
GO:0036503 |
ERAD pathway |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.