Overview
| Uniprot ID | A0A8I5Y5C7 |
|---|
| Protein Name | Poly(U)-binding-splicing factor PUF60 |
|---|
| Gene Name | Puf60 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 452 |
ARHMVMQKLLRKQES |
Function
DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with RO60. Binds to poly(U) RNA
Protein Sequence
10
MQVNGQQGGG
20
SEPAAAAAAA
30
AAAVVAAGDK
40
WKPPQGTESI
50
KMENGQSTGT
60
KLGLPPLTPE
70
QQEALQKAKK
80
YAMEQSIKSV
90
LVKQTIAHQQ
100
QQLTNLQMAA
110
VTMGFGDPLS
120
PLQSMAAQRQ
130
RALAIMCRVY
140
VGSIYYELGE
150
DTIRQAFAPF
160
GPIKSIDMSW
170
DSVTMKHKGF
180
AFVEYEVPEA
190
AQLALEQMNS
200
VMLGGRNIKV
210
GRPSNIGQAQ
220
PIIDQLAEEA
230
RAFNRIYVAS
240
VHQDLSDDDI
250
KSVFEAFGKI
260
KSCTLARDPT
270
TGKHKGYGFI
280
EYEKAQSSQD
290
AVSSMNLFDL
300
GGQYLRVGKA
310
VTPPMPLLTP
320
ATPGGLPPAA
330
AVAAAAATAK
340
ITAQEAVAGA
350
AVLGTLATPG
360
LVSPALTLAQ
370
PLGALPQAVM
380
AAQAPGVITG
390
VTPARPPIPV
400
TIPSVGVVNP
410
ILASPPTLGL
420
LEPKKEKEEE
430
ELFPESERPE
440
MLSEQEHMSI
450
SGSSARHMVM
460
QKLLRKQEST
470
VMVLRNMVDP
480
KDIDDDLEGE
490
VTEECGKFGA
500
VNRVIIYQEK
510
QGEEEDAEII
520
VKIFVEFSMA
530
SETHKAIQAL
540
NGRWFGGRKV
550
VAEVYDQERF
DNSDLSA
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:1990904 |
ribonucleoprotein complex |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0003723 |
RNA binding |
| Biological Process |
GO:0006915 |
apoptotic process |
| Biological Process |
GO:0006351 |
DNA-templated transcription |
| Biological Process |
GO:0006397 |
mRNA processing |
| Biological Process |
GO:0000381 |
regulation of alternative mRNA splicing, via spliceosome |
| Biological Process |
GO:0008380 |
RNA splicing |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.