Search Results

Overview

Uniprot ID	A0A8I5Y7L4
Protein Name	DNA topoisomerase 2
Gene Name	Top2b
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
1202	ILAGMSGKAVKGKVG

Function

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand

Protein Sequence

10 MAKSSLAGVD 20 GALTWVNNAA 30 KKEELETANK 40 NDSSKKLSVE 50 RVYQKKTQLE 60 HILLRPDTYI 70 GSVEPLTQLM 80 WVYDEDVGMN 90 CREVTFVPGL 100 YKIFDEILVN 110 AADNKQRDKN 120 MTCIKVSIDP 130 ESNIISIWNN 140 GKGIPVVEHK 150 VEKVYVPALI 160 FGQLLTSSNY 170 DDDEKKVTGG 180 RNGYGAKLCN 190 IFSTKFTVET 200 ACKEYKHSFK 210 QTWMNNMMKT 220 SEAKIKHFDG 230 EDYTCITFQP 240 DLAKFKMEKL 250 DKDIVALMTR 260 RAYDLAGSCK 270 GVKVMFNGKK 280 LPVNGFRSYV 290 DLYVKDKLDE 300 TGVALKVIHE 310 LANERWDVCL 320 TLSEKGFQQI 330 SFVNSIATTK 340 GGRHVDYVVD 350 QVVGKLIEVV 360 KKKNKAGVSV 370 KPFQVKNHIW 380 VFINCLIENP 390 TFDSQTKENM 400 TLQPKSFGSK 410 CQLSEKFFKA 420 ASNCGIVESI 430 LNWVKFKAQT 440 QLNKKCSSVK 450 YSKIKGIPKL 460 DDANDAGGKH 470 SLECTLILTE 480 GDSAKSLAVS 490 GLGVIGRDRY 500 GVFPLRGKIL 510 NVREASHKQI 520 MENAEINNII 530 KIVGLQYKKS 540 YDDAESLKTL 550 RYGKIMIMTD 560 QDQDGSHIKG 570 LLINFIHHNW 580 PSLLKHGFLE 590 EFITPIVKAS 600 KNKQELSFYS 610 IPEFDEWKKH 620 IENQKAWKIK 630 YYKGLGTSTA 640 KEAKEYFADM 650 ERHRILFRYA 660 GPEDDAAITL 670 AFSKKKIDDR 680 KEWLTNFMED 690 RRQRRLHGLP 700 EQFLYGTATK 710 HLTYNDFINK 720 ELILFSNSDN 730 ERSIPSLVDG 740 FKPGQRKVLF 750 TCFKRNDKRE 760 VKVAQLAGSV 770 AEMSAYHHGE 780 QALMMTIVNL 790 AQNFVGSNNI 800 NLLQPIGQFG 810 TRLHGGKDAA 820 SPRYIFTMLS 830 SLARLLFPAV 840 DDNLLKFLYD 850 DNQRVEPEWY 860 IPIIPMVLIN 870 GAEGIGTGWA 880 CKLPNYDARE 890 IVNNVRRMLE 900 GLDPHPMLPN 910 YKNFKGTIQE 920 LGQNQYAVSG 930 EIFVVDRNTV 940 EITELPVRTW 950 TQVYKEQVLE 960 PMLNGTDKTP 970 ALISDYKEYH 980 TDTTVKFVVK 990 MTEEKLAQAE 1000 AAGLHKVFKL 1010 QTTLTCNSMV 1020 LFDHMGCLKK 1030 YETVQDILKE 1040 FFDLRLSYYG 1050 LRKEWLVGML 1060 GAESTKLNNQ 1070 ARFILEKIQG 1080 KITIENRSKK 1090 DLIQMLVQRG 1100 YESDPVKAWK 1110 EAQEKAAEEE 1120 DAQNPHDDSS 1130 SDSGTPSGPD 1140 FNYILNMSLW 1150 SLTKEKVEEL 1160 IKQRDTKGRE 1170 VNDLKRKSPS 1180 DLWKEDLAAF 1190 VEELDKVEAQ 1200 EREDILAGMS 1210 GKAVKGKVGK 1220 AKVKKLQLEE 1230 TMPSPYGRRI 1240 VPEITAMKAD 1250 ASRKLLKKKK 1260 GDPDTTVVKV 1270 EFDEEFSGTP 1280 AEGTGEETLT 1290 PSAPVNKGPK 1300 PKREKKEPGT 1310 RVRKTPASTG 1320 KPNTKKVKKR 1330 NPWSDDESKS 1340 ESDLEEAEPV 1350 VIPRDSLLRR 1360 AAAERPKYTF 1370 DFSEEEEDDA 1380 DDDDDNNDLE 1390 ELKVKASPIT 1400 NDGEDEFVPS 1410 DGIDKDEYAF 1420 SPGKSKATPE 1430 KSSHDKKSQD 1440 FGNLFSFPSY 1450 SQKSEDDSAK 1460 FDSNEEDTTS 1470 VFAPSFGLKQ 1480 TDKVPSQTVA 1490 AKKGKAPSDA 1500 AAPKAKRAPR 1510 QRKVVEPANS 1520 DSDSELGNIP 1530 KKTAAPKGKG 1540 RGAKKRKASG 1550 SENEGDYNPG 1560 RKPSKTASKK 1570 PKKTSFDQDS 1580 DVDIFPSDFT 1590 SEPPALPRTG 1600 RARKEVKYFA 1610 ESDEEEDVDF AMFN

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0000792	heterochromatin
Cellular Component	GO:0005730	nucleolus
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0003682	chromatin binding
Molecular Function	GO:0003677	DNA binding
Molecular Function	GO:0003918	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
Molecular Function	GO:0046872	metal ion binding
Molecular Function	GO:0043021	ribonucleoprotein complex binding
Biological Process	GO:0007409	axonogenesis
Biological Process	GO:0030183	B cell differentiation
Biological Process	GO:0071318	cellular response to ATP
Biological Process	GO:0070301	cellular response to hydrogen peroxide
Biological Process	GO:0090398	cellular senescence
Biological Process	GO:0006265	DNA topological change
Biological Process	GO:0030900	forebrain development
Biological Process	GO:0001764	neuron migration
Biological Process	GO:2001034	positive regulation of double-strand break repair via nonhomologous end joining
Biological Process	GO:0045870	positive regulation of single stranded viral RNA replication via double stranded DNA intermediate
Biological Process	GO:0000712	resolution of meiotic recombination intermediates
Biological Process	GO:0000819	sister chromatid segregation

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.