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Overview

Uniprot ID	A0A8I5Y9J0
Protein Name	Intersectin-1
Gene Name	Itsn1
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
1050	EGSGTAGKTGSLGKK

Function

Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery. Acts as a guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex. Plays a role in the assembly and maturation of clathrin-coated vesicles. Recruits FCHSD2 to clathrin-coated pits. Involved in endocytosis of activated EGFR, and probably also other growth factor receptors. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2. Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways. In chromaffin cells, required for normal exocytosis of catecholamines. Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones. Inhibits ARHGAP31 activity toward RAC1

Protein Sequence

10 ILDIWAITVE 20 ERAKHDQQFQ 30 SLKPISGFIT 40 GDQARNFFFQ 50 SGLPQPVLAQ 60 IWALADMNKD 70 GRMDQVEFSI 80 AMKLIKLKLQ 90 GYQLPPALPP 100 VMKQQPAAIS 110 SAPAFGIGGM 120 AGMPPLTAVA 130 PVPMGSIPVV 140 GMSPPLVSSV 150 PQAAVPPLAN 160 GAPPVIQPLP 170 AFAHPAATLP 180 KSSSFSRSGP 190 GSQLNTKLQK 200 AQSFDVASAP 210 AAAEWAVPQS 220 SRLKYRQLFN 230 SHDKTMSGHL 240 TGPQARTILM 250 QSSLPQAQLA 260 SIWNLSDIDQ 270 DGKLTAEEFI 280 LAMHLIDVAM 290 SGQPLPPVLP 300 PEYIPPSFRR 310 VRSGSGMSVI 320 SSSSADQRLP 330 EEPSSEDEQQ 340 VEKKLPVTFE 350 DKKRENFERG 360 NLELEKRRQA 370 LLEQQRKEQE 380 RLAQLERAEQ 390 ERKERERQEQ 400 ERKRQLELEK 410 QLEKQRELER 420 QREEERRKEI 430 ERREAAKREL 440 ERQRQLEWER 450 NRRQELLTQR 460 NKDQEGIVVL 470 KARRKTLEFE 480 LEALNDKKHQ 490 LEGKLQDIRC 500 RLATQRQEIE 510 STNKSRELRI 520 AEITHLQQQL 530 QESQQMLGRL 540 IPEKQILSDQ 550 LKQVQQNSLH 560 RDSLLTLKRA 570 LEAKELARQQ 580 LREQLDEVEK 590 ETRSKLQEID 600 VFNNQLKELR 610 EIHSKQQLQK 620 QRSIEAERLK 630 QKEQERKSLE 640 LEKQKEEGQR 650 RVQERDKQWQ 660 EHVQQEEQQR 670 PRKPHEEDKL 680 KREDSVKKKE 690 AEERAKPEVQ 700 DKQSRLFHPH 710 QEPAKPAQAP 720 WPTTEKGPLT 730 ISAQESAKVV 740 YYRALYPFES 750 RSHDEITIQP 760 GDIVMVDESQ 770 TGEPGWLGGE 780 LKGKTGWFPA 790 NYAEKIPENE 800 IPTPAKPVTD 810 LTSAPAPKLA 820 LRETPAPLPV 830 TSSEPSTTPN 840 NWADFSSTWP 850 SSTNEKPETD 860 NWDTWAAQPS 870 LTVPSAGQLR 880 QRSAFTPATA 890 TGSSPSPVLG 900 QGEKVEGLQA 910 QALYPWRAKK 920 DNHLNFNKSD 930 VITVLEQQDM 940 WWFGEVQGQK 950 GWFPKSYVKL 960 ISGPVRKSTS 970 IDTGPTEAPS 980 SLKRVASPAA 990 KPAIPGEEFV 1000 AMYTYESSEH 1010 GDLTFQQGDV 1020 IVVTKKDGDW 1030 WTGTVGETSG 1040 VFPSNYVRLK 1050 DSEGSGTAGK 1060 TGSLGKKPEI 1070 AQVIASYTAT 1080 GPEQLTLAPG 1090 QLILIRKKNP 1100 GGWWEGELQA 1110 RGKKRQIGWF 1120 PANYVKLLSP 1130 GTSKITPTEL 1140 PKTAVQPAVC 1150 QVIGMYDYTA 1160 QNDDELAFSK 1170 GQIINVLSKE 1180 DPDWWKGEVS 1190 GQVGLFPSNY 1200 VKLTTDMDPS 1210 QQWCSDLHLL 1220 DMLTPTERKR 1230 QGYIHELIVT 1240 EENYVNDLQL 1250 VTEIFQKPLT 1260 ESELLTEKEV 1270 AMIFVNWKEL 1280 IMCNIKLLKA 1290 LRVRKKMSGE 1300 KMPVKMIGDI 1310 LSAQLPHMQP 1320 YIRFCSCQLN 1330 GAALIQQKTD 1340 EAPDFKEFVK 1350 RLAMDPRCKG 1360 MPLSSFILKP 1370 MQRVTRYPLI 1380 IKNILENTPE 1390 NHPDHSHLKH 1400 ALEKAEELCS 1410 QVNEGVREKE 1420 NSDRLEWIQA 1430 HVQCEGLSEQ 1440 LVFNSVTNCL 1450 GPRKFLHSGK 1460 LYKAKSNKEL 1470 YGFLFNDFLL 1480 LTQITKPLGS 1490 SSTDKVFSPK 1500 SNLQYKMYKT 1510 PIFLNEVLVK 1520 LPTDPSGDEP 1530 IFHISHIDRV 1540 YTLRAESINE 1550 RTAWVQKIKA 1560 ASELYIETEK 1570 KKREKAYLVR 1580 SQRATGIGRL 1590 MVNVVEGIEL 1600 KPCRSHGKSN 1610 PYCEVTMGSQ 1620 CHITKTIQDT 1630 LNPKWNSNCQ 1640 FFIRDLEQEV 1650 LCITVFERDQ 1660 FSPDDFLGRT 1670 EIRVADIKKD 1680 QGSKGPVTKC 1690 LLLHEVPTGE 1700 IVVRLDLQLF DEP

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005905	clathrin-coated pit
Cellular Component	GO:0030027	lamellipodium
Cellular Component	GO:0043005	neuron projection
Cellular Component	GO:0005635	nuclear envelope
Cellular Component	GO:0005886	plasma membrane
Cellular Component	GO:0055037	recycling endosome
Cellular Component	GO:0045202	synapse
Molecular Function	GO:0005509	calcium ion binding
Molecular Function	GO:0005085	guanyl-nucleotide exchange factor activity
Biological Process	GO:0006897	endocytosis
Biological Process	GO:0006887	exocytosis
Biological Process	GO:0035556	intracellular signal transduction
Biological Process	GO:0015031	protein transport
Biological Process	GO:0010646	regulation of cell communication
Biological Process	GO:0023051	regulation of signaling

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.