Overview
| Uniprot ID | A0A8I5ZWG5 |
| Protein Name | Presequence protease, mitochondrial |
| Gene Name | Pitrm1 |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 1010 |
PENSKLAKDPSWIIR |
Function
Metalloendopeptidase of the mitochondrial matrix that functions in peptide cleavage and degradation rather than in protein processing. Has an ATP-independent activity. Specifically cleaves peptides in the range of 5 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. Degrades the transit peptides of mitochondrial proteins after their cleavage. Also degrades other unstructured peptides. It is also able to degrade amyloid-beta protein 40, one of the peptides produced by APP processing, when it accumulates in mitochondrion. It is a highly efficient protease, at least toward amyloid-beta protein 40. Cleaves that peptide at a specific position and is probably not processive, releasing digested peptides intermediates that can be further cleaved subsequently. It is also able to degrade amyloid-beta protein 42
Protein Sequence
10
MVHHKVWREK
20
SDQACDRALQ
30
YKVGEKIHGF
40
TVNQVTPVPE
50
LFLTAVKLSH
60
DNTGARYLHL
70
AREDNNNLFS
80
VQFRTTPMDS
90
TGVPHVLEHT
100
VLCGSQKYPC
110
RDPFFKMLNR
120
SLSTFMNAFT
130
ASDYTMYPFS
140
TQNPKDFQNL
150
LSVYLDATFF
160
PCLRELDFWQ
170
EGWRLEHEDP
180
SDPQTPLIFK
190
GVVFNEMKGA
200
FTDNERIFSQ
210
HLQNKLLPDH
220
TYSVVSGGDP
230
LCIPELTWEQ
240
LKQFHTTHYH
250
PSNARFFTYG
260
NFPLEDHLKQ
270
IHEEALSKFQ
280
KMEESTAVPA
290
QKYWDKPREF
300
HITCGPDSLA
310
TDATKQTTVS
320
VSFLLPDITN
330
TFEAFTLNLL
340
SSLLISGPNS
350
PFYKALIESG
360
LGTDFSPDVG
370
YNGYTREAYF
380
SVGLQGIAEN
390
DVKTVRELVD
400
RTIEEVIEKG
410
FEDDQIEALL
420
HKIEIQMKHQ
430
SASFGMALTS
440
YIASCWNHDG
450
DPVELLQMGS
460
QLTKFRKCLK
470
ENPKFLQEKV
480
EQYFKNNPHR
490
LTLSMKPDDR
500
YYEKQTQMET
510
EKLEQKVNSL
520
SQADKKQIYE
530
KGLELQKQQS
540
KHQDASCLPA
550
LKVSDIEPTM
560
PFTKFDIALS
570
AGDVPVQYCP
580
QPTNGIVYFR
590
AFSSLNTLPE
600
ELRPFVPLFC
610
TVLTKLGCGI
620
LNYREQAQQI
630
ELKTGGMTVT
640
PHVLPDDSQL
650
DTYEQGVLFS
660
SLCLERNLPD
670
MMHLWSEIFN
680
NPCFEEEEHF
690
KVLVRMSAQE
700
LSNGIPDSGH
710
LYAALRAGKT
720
LTPAGDLQET
730
FSGMDQVKVM
740
KRIAEMTDIK
750
PILRKLPRIK
760
KYLLNCDNMR
770
CSVNATPQQM
780
PQAEKEVENF
790
LRNVGRSKKE
800
RKPVRPHIVE
810
KPTPSGPSGG
820
AHADGSQIIR
830
KLITDPTFKP
840
CQMKTHFVLP
850
FPVNYVGECV
860
RTVPYADPDH
870
ASLKILARLM
880
TAKFLHTEIR
890
EKGGAYGGGA
900
KVTHTGIFTL
910
YSYRDPNSIE
920
TLQSFGKAID
930
WAKSGKFTQQ
940
DIDEAKLSVF
950
SAVDSPVAPS
960
DKGMDHFLYG
970
LSDEMKQTYR
980
EQLFAVTHDK
990
LTSVSHKYLG
1000
IGKSTHGLAI
1010
LGPENSKLAK
DPSWIIR
Gene Ontology
| Classification |
GO ID |
Description |
| Biological Process |
GO:0006508 |
proteolysis |
| Cellular Component |
GO:0005759 |
mitochondrial matrix |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0008237 |
metallopeptidase activity |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.