Search Results

Overview

Uniprot ID	A0A8I6A8E8
Protein Name	Probable ATP-dependent RNA helicase DDX46
Gene Name	Ddx46
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
1027	NSYQPTNKGRYKVL*

Function

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, DDX46 plays essential roles during assembly of pre-spliceosome and proofreading of the branch site

Protein Sequence

10 MGIEPRAFHY 20 RKRSASRGRS 30 GSRSRSRSPS 40 DKRSKRGDDR 50 RSRSRDRDRR 60 RERSRSRDKR 70 RSRSRDRKRL 80 RRSRSRERDR 90 SRERRRSRSR 100 DRRRSRSRSR 110 GRRSRSSSPG 120 SKSKKAENRS 130 RSKEKAEGGD 140 SSKEKKKDKD 150 DKEDEKEKDA 160 GNFDQNKLEE 170 EMRKRKERVE 180 KWREEQRKKA 190 MENIGELKKE 200 IEEMKQGKKW 210 SLEDDDDDED 220 DPAEAEKEGN 230 EMEDEELDPL 240 DAYMEEVKEE 250 VKKFNMRSVK 260 GGAGNEKKSG 270 PTVTKVVTVV 280 TTKKAVVDAD 290 KKKGELMEND 300 QDAMEYSSEE 310 EEVDLQTALT 320 GYQTKQRKLL 330 EPVDHGKIEY 340 EPFRKNFYVE 350 VPELAKMSQE 360 EVNVFRLEME 370 GITVKGKGCP 380 KPIKSWVQCG 390 ISMKILNSLK 400 KHGYEKPTPI 410 QTQAIPAIMS 420 GRDLIGIAKT 430 GSGKTIAFLL 440 PMFRHIMDQR 450 SLEEGEGPIA 460 VIMTPTRELA 470 LQITKECKKF 480 SKTLGLRVVC 490 VYGGTGISEQ 500 IAELKRGAEI 510 IVCTPGRMID 520 MLAANSGRVT 530 NLRRVTYVVL 540 DEADRMFDMG 550 FEPQVMRIVD 560 NVRPDRQTVM 570 FSATFPRAME 580 ALARRILSKP 590 IEVQVGGRSV 600 VCSDVEQQVI 610 VIEEEKKFLK 620 LLELLGHYQE 630 SGSVIIFVDK 640 QEHADGLLKD 650 LMRASYPCMS 660 LHGGIDQYDR 670 DSIINDFKNG 680 TCKLLVATSV 690 AARGLDVKHL 700 ILVVNYSCPN 710 HYEDYVHRAG 720 RTGRAGNKGY 730 AYTFITEDQA 740 RYAGDIIKAL 750 ELSGTAVPPD 760 LEKLWSDFKD 770 QQKAEGKIIK 780 KSSGFSGKGF 790 KFDETEQALA 800 NERKKLQKAA 810 LGLQDSDDED 820 AAVDIDEQIE 830 SMFNSKKRVK 840 DMAAPGTSSA 850 PAPTAGNAEK 860 LEIAKRLALR 870 INAQKNLGIE 880 SQDVMQQATN 890 AILRGGPLLA 900 PTVSAKTIAE 910 QLAEKINAKL 920 NYVPLEKQEE 930 ERQEGGQSES 940 FKRYEEELEI 950 NDFPQTARWK 960 VTSKEALQRI 970 SEYSEAAITI 980 RGTYFPPGKE 990 PKEGERKIYL 1000 AIESANELAV 1010 QKAKAEITRL 1020 IKEELIRLQN 1030 SYQPTNKGRY KVL

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0015030	Cajal body
Cellular Component	GO:0016607	nuclear speck
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0016787	hydrolase activity
Molecular Function	GO:0003676	nucleic acid binding
Molecular Function	GO:0003724	RNA helicase activity

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.