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Overview

Uniprot IDA0A8I6A8S5
Protein NameEukaryotic translation initiation factor 3 subunit A
Gene NameEif3a
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
294 HLSREMRKNLTQDEM

Function

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression

Protein Sequence

10 MNPFHLVGKK 20 QPALDVLYDV 30 MKSKKHRTWQ 40 KIHEPIMLKY 50 LELCVDLRKS 60 HLAKEGLYQY 70 KNICQQVNIK 80 SLEDVVRAYL 90 KLAEEKTEAA 100 KEESQQMVLD 110 IEDLDNIQTP 120 ESVLLSAVSG 130 EDTQDRTDRL 140 LLTPWVKFLW 150 ESYRQCLDLL 160 RNNSRVERLY 170 HDIAQQAFKF 180 CLQYTRKAEF 190 RKLCDNLRMH 200 LSQIQRHHNQ 210 STAINLNNPE 220 SQSMHLETRL 230 VQLDSAISME 240 LWQEAFKAVE 250 DIHGLFSLSK 260 KPPKPQLMAN 270 YYNKVSTVFW 280 KSGNALFHAS 290 TLHRLYHLSR 300 EMRKNLTQDE 310 MQRMSTRVLL 320 ATLSIPITPE 330 RTDIARLLDM 340 DGIIVEKQRR 350 LATLLGLQAP 360 PTRIGLINDM 370 VRFNVLQYVV 380 PEVKDLYNWL 390 EVEFNPLKLC 400 ERVTKVLNWV 410 REQPEKEPEL 420 QQYVPQLQNN 430 TILRLLQQVA 440 QIYQSIEFSR 450 LTSLVPFVDA 460 FQLERAIVDA 470 ARHCDLQVRI 480 DHTSRTLSFG 490 SDLNYATRED 500 APIGPHLQSM 510 PSEQIRNQLT 520 AMSSVLAKAL 530 EVIKPAHILQ 540 EKEEQHQLAV 550 TAYIKNSRKE 560 HQRILARRQT 570 IEERKERLES 580 LNIQREKEEL 590 EQREAELQKV 600 RKAEEERLRQ 610 EAKEREKERI 620 LQEHEQIKKK 630 TVRERLEQIK 640 KTELGAKAFK 650 DIDIEDLEEL 660 DPDFIMAKQV 670 EQLEKEKKEL 680 QERLKNQEKK 690 IDYFERAKRL 700 EEIPLIKSAY 710 EEQRVKDMDL 720 WEQQEEERIT 730 TMQLEREKAL 740 EHKNRMSRML 750 EDRDLFVMRL 760 KAARQSVYEE 770 KLKQFEERLA 780 EERHNRLEER 790 KRQRKEERKI 800 TYYREKEEEE 810 QRRAEEQMLK 820 EREERERAER 830 AKREEELREY 840 QERVKKLEEV 850 ERKKRQRELE 860 IEERERRREE 870 ERRLGEDPLS 880 RKDSRWGDRD 890 SEGTWRKGPE 900 ADSEWRRGPP 910 EKEWRRETRD 920 DERPHRRDED 930 RLRRLGGDDE 940 ERESSLRPDD 950 DRIPRRGLDD 960 DRGPRRGPDE 970 DRFSRRGTDD 980 DRPSWRNADD 990 DRPPRRIGDD 1000 DRGSWRHTDD 1010 DRPPRRGLDD 1020 DRPPRRGLDD 1030 ERGSWRTAEE 1040 DRGPRRGMDD 1050 DRGPRRGGAD 1060 DERSSWRNAD 1070 DDRGPRRGMD 1080 DDRGPRRGLD 1090 DDRGPWRNAA 1100 EDRISRRGAD 1110 DDRGPWRNMD 1120 DDRVPRRGDD 1130 ARPGPWRPFV 1140 KPGGWREKEK 1150 AREESWGPPR 1160 ESRPPEEREW 1170 DRDKEKDRDN 1180 QDREENDKDL 1190 ERDRDRERDG 1200 DREDRFRRPR 1210 DEGGWRRGPA 1220 EESSSWRDSS 1230 RRDDRDRDDR 1240 RRDRDDRRDL 1250 RDLRDRRDLR 1260 DDRDRRGPPL 1270 RSEREEASSW 1280 RRTDDRKDDR 1290 TEERDPPRRV 1300 PPPTLSRERE 1310 RERDREGEKE 1320 KASWRAEKDR 1330 ESLRRTKNET 1340 DEDGWTTVRR

Gene Ontology

Classification GO ID Description
Cellular Component GO:0016282 eukaryotic 43S preinitiation complex
Cellular Component GO:0033290 eukaryotic 48S preinitiation complex
Cellular Component GO:0005852 eukaryotic translation initiation factor 3 complex
Cellular Component GO:0043226 organelle
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003743 translation initiation factor activity
Biological Process GO:0001732 formation of cytoplasmic translation initiation complex

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.