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Overview

Uniprot IDA0A8I6AAD9
Protein NameSuccinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial
Gene NameSuclg1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
289 GAIIAGGKGGAKEKI
29 GSYTASRKNIYIDKN
35 RKNIYIDKNTKVICQ
38 IYIDKNTKVICQGFT
47 ICQGFTGKQGTFHSQ
71 VGGTTPGKGGKKHLG
75 TPGKGGKKHLGLPVF
86 LPVFNTVKEAKEKTG

Function

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits. Also able to act as an itaconyl- and malyl-CoA synthetase

Protein Sequence

10 SPGSGCLALL 20 YFWVQQNGIR 30 HGSYTASRKN 40 IYIDKNTKVI 50 CQGFTGKQGT 60 FHSQQALEYG 70 TKLVGGTTPG 80 KGGKKHLGLP 90 VFNTVKEAKE 100 KTGATASVIY 110 VPPPFAAAAI 120 NEAIDAEIPL 130 VVCITEGIPQ 140 QDMVRVKHKL 150 TRQGKTRLIG 160 PNCPGIINPG 170 ECKIGIMPGH 180 IHKKGRIGIV 190 SRSGTLTYEA 200 VHQTTQVGLG 210 QSLCIGIGGD 220 PFNGTNFIDC 230 LDVFLKDPAT 240 EGIVLIGEIG 250 GHAEENAAEF 260 LKEHNSGPKA 270 KPVVSFIAGI 280 TAPPGRRMGH 290 AGAIIAGGKG 300 GAKEKISALQ 310 SAGVIVSMSP 320 AQLGTCMYKV KFKNSALL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0045244 succinate-CoA ligase complex (GDP-forming)
Molecular Function GO:0000166 nucleotide binding
Molecular Function GO:0004775 succinate-CoA ligase (ADP-forming) activity
Molecular Function GO:0004776 succinate-CoA ligase (GDP-forming) activity
Biological Process GO:0006099 tricarboxylic acid cycle

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.