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Overview

Uniprot IDA0A8I6ABA1
Protein NameMethyl-CpG-binding protein 2
Gene NameMecp2
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
200 GSGTGRPKAAASEGV
210 ASEGVQVKRVLEKSP
22 DQDLQGLKEKPLKFK
24 DLQGLKEKPLKFKKV
249 SAQVMVIKRPGRKRK
266 ADPQAIPKKRGRKPG
271 IPKKRGRKPGSVVAA
304 QETVLPIKKRKTRET
331 LVSTLGEKSGKGLKT
465 HRGEGERKDIVSSSM

Function

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC)

Protein Sequence

10 SDSGMLRSQE 20 EKSEDQDLQG 30 LKEKPLKFKK 40 VKKDKKEDKE 50 GKHEPLQPSA 60 HHSAEPAEAG 70 KAETSESSGS 80 APAVPEASAS 90 PKQRRSIIRD 100 RGPMYDDPTL 110 PEGWTRKLKQ 120 RKSGRSAGKY 130 DVYLINPQGK 140 AFRSKVELIA 150 YFEKVGDTSL 160 DPNDFDFTVT 170 GRGSPSRREQ 180 KPPKKPKSPK 190 APGTGRGRGR 200 PKGSGTGRPK 210 AAASEGVQVK 220 RVLEKSPGKL 230 LVKMPFQASP 240 GGKGEGGGAT 250 TSAQVMVIKR 260 PGRKRKAEAD 270 PQAIPKKRGR 280 KPGSVVAAAA 290 AEAKKKAVKE 300 SSIRSVQETV 310 LPIKKRKTRE 320 TVSIEVKEVV 330 KPLLVSTLGE 340 KSGKGLKTCK 350 SPGRKSKESS 360 PKGRSSSASS 370 PPKKEHHHHH 380 HHAESPKAPM 390 PLLPPPPPPE 400 PQSSEDPISP 410 PEPQDLSSSI 420 CKEEKMPRAG 430 SLESDGCPKE 440 PAKTQPMVAA 450 AATTTTTTTT 460 TVAEKYKHRG 470 EGERKDIVSS 480 SMPRPNREEP 490 VDSRTPVTER VS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005813 centrosome
Cellular Component GO:0005829 cytosol
Cellular Component GO:0000792 heterochromatin
Cellular Component GO:0005654 nucleoplasm
Molecular Function GO:0010385 double-stranded methylated DNA binding
Molecular Function GO:0140566 histone reader activity
Molecular Function GO:0008327 methyl-CpG binding
Molecular Function GO:0140693 molecular condensate scaffold activity
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:1990841 promoter-specific chromatin binding
Molecular Function GO:0035197 siRNA binding
Molecular Function GO:0003714 transcription corepressor activity
Biological Process GO:0008306 associative learning
Biological Process GO:0048468 cell development
Biological Process GO:0021549 cerebellum development
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0016525 negative regulation of angiogenesis
Biological Process GO:0043537 negative regulation of blood vessel endothelial cell migration
Biological Process GO:0044027 negative regulation of gene expression via chromosomal CpG island methylation
Biological Process GO:0051151 negative regulation of smooth muscle cell differentiation
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0030182 neuron differentiation
Biological Process GO:0007219 Notch signaling pathway
Biological Process GO:0090063 positive regulation of microtubule nucleation
Biological Process GO:0043576 regulation of respiratory gaseous exchange
Biological Process GO:0035176 social behavior

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.