Search Results

Overview

Uniprot ID	A0A8I6ARZ8
Protein Name	Intersectin-1
Gene Name	Itsn1
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
1065	EGSGTAGKTGSLGKK

Function

Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery. Acts as a guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex. Plays a role in the assembly and maturation of clathrin-coated vesicles. Recruits FCHSD2 to clathrin-coated pits. Involved in endocytosis of activated EGFR, and probably also other growth factor receptors. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2. Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways. In chromaffin cells, required for normal exocytosis of catecholamines. Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones. Inhibits ARHGAP31 activity toward RAC1

Protein Sequence

10 MAQFPTPFGG 20 SLDIWAITVE 30 ERAKHDQQFQ 40 SLKPISGFIT 50 GDQARNFFFQ 60 SGLPQPVLAQ 70 IWALADMNKD 80 GRMDQVEFSI 90 AMKLIKLKLQ 100 GYQLPPALPP 110 VMKQQPAAIS 120 SAPAFGIGGM 130 AGMPPLTAVA 140 PVPMGSIPVV 150 GMSPPLVSSV 160 PQAAVPPLAN 170 GAPPVIQPLP 180 AFAHPAATLP 190 KSSSFSRSGP 200 GSQLNTKLQK 210 AQSFDVASAP 220 AAAEWAVPQS 230 SRLKYRQLFN 240 SHDKTMSGHL 250 TGPQARTILM 260 QSSLPQAQLA 270 SIWNLSDIDQ 280 DGKLTAEEFI 290 LAMHLIDVAM 300 SGQPLPPVLP 310 PEYIPPSFRR 320 VRSGSGMSVI 330 SSSSADQRLP 340 EEPSSEDEQQ 350 VEKKLPVTFE 360 DKKRENFERG 370 NLELEKRRQA 380 LLEQQRKEQE 390 RLAQLERAEQ 400 ERKERERQEQ 410 ERKRQLELEK 420 QLEKQRELER 430 QREEERRKEI 440 ERREAAKREL 450 ERQRQLEWER 460 NRRQELLTQR 470 NKDQEGIVVL 480 KARRKTLEFE 490 LEALNDKKHQ 500 LEGKLQDIRC 510 RLATQRQEIE 520 STNKSRELRI 530 AEITHLQQQL 540 QESQQMLGRL 550 IPEKQILSDQ 560 LKQVQQNSLH 570 RDSLLTLKRA 580 LEAKELARQQ 590 LREQLDEVEK 600 ETRSKLQEID 610 VFNNQLKELR 620 EIHSKQQLQK 630 QRSIEAERLK 640 QKEQERKSLE 650 LEKQKEEGQR 660 RVQERDKQWQ 670 EHVQQEEQQR 680 PRKPHEEDKL 690 KREDSVKKKE 700 AEERAKPEVQ 710 DKQSRLFHPH 720 QEPAKPAQAP 730 WPTTEKGPLT 740 ISAQESAKVV 750 YYRALYPFES 760 RSHDEITIQP 770 GDIVMVKGEW 780 VDESQTGEPG 790 WLGGELKGKT 800 GWFPANYAEK 810 IPENEIPTPA 820 KPVTDLTSAP 830 APKLALRETP 840 APLPVTSSEP 850 STTPNNWADF 860 SSTWPSSTNE 870 KPETDNWDTW 880 AAQPSLTVPS 890 AGQLRQRSAF 900 TPATATGSSP 910 SPVLGQGEKV 920 EGLQAQALYP 930 WRAKKDNHLN 940 FNKSDVITVL 950 EQQDMWWFGE 960 VQGQKGWFPK 970 SYVKLISGPV 980 RKSTSIDTGP 990 TEAPSSLKRV 1000 ASPAAKPAIP 1010 GEEFVAMYTY 1020 ESSEHGDLTF 1030 QQGDVIVVTK 1040 KDGDWWTGTV 1050 GETSGVFPSN 1060 YVRLKDSEGS 1070 GTAGKTGSLG 1080 KKPEIAQVIA 1090 SYTATGPEQL 1100 TLAPGQLILI 1110 RKKNPGGWWE 1120 GELQARGKKR 1130 QIGWFPANYV 1140 KLLSPGTSKI 1150 TPTELPKTAV 1160 QPAVCQVIGM 1170 YDYTAQNDDE 1180 LAFSKGQIIN 1190 VLSKEDPDWW 1200 KGEVSGQVGL 1210 FPSNYVKLTT 1220 DMDPSQQWCS 1230 DLHLLDMLTP 1240 TERKRQGYIH 1250 ELIVTEENYV 1260 NDLQLVTEIF 1270 QKPLTESELL 1280 TEKEVAMIFV 1290 NWKELIMCNI 1300 KLLKALRVRK 1310 KMSGEKMPVK 1320 MIGDILSAQL 1330 PHMQPYIRFC 1340 SCQLNGAALI 1350 QQKTDEAPDF 1360 KEFVKRLAMD 1370 PRCKGMPLSS 1380 FILKPMQRVT 1390 RYPLIIKNIL 1400 ENTPENHPDH 1410 SHLKHALEKA 1420 EELCSQVNEG 1430 VREKENSDRL 1440 EWIQAHVQCE 1450 GLSEQLVFNS 1460 VTNCLGPRKF 1470 LHSGKLYKAK 1480 SNKELYGFLF 1490 NDFLLLTQIT 1500 KPLGSSSTDK 1510 VFSPKSNLQY 1520 KMYKTPIFLN 1530 EVLVKLPTDP 1540 SGDEPIFHIS 1550 HIDRVYTLRA 1560 ESINERTAWV 1570 QKIKAASELY 1580 IETEKKKREK 1590 AYLVRSQRAT 1600 GIGRLMVNVV 1610 EGIELKPCRS 1620 HGKSNPYCEV 1630 TMGSQCHITK 1640 TIQDTLNPKW 1650 NSNCQFFIRD 1660 LEQEVLCITV 1670 FERDQFSPDD 1680 FLGRTEIRVA 1690 DIKKDQGSKG 1700 PVTKCLLLHE 1710 VPTGEIVVRL DLQLFDEP

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005905	clathrin-coated pit
Cellular Component	GO:0030027	lamellipodium
Cellular Component	GO:0043005	neuron projection
Cellular Component	GO:0005635	nuclear envelope
Cellular Component	GO:0005886	plasma membrane
Cellular Component	GO:0055037	recycling endosome
Cellular Component	GO:0045202	synapse
Molecular Function	GO:0005509	calcium ion binding
Molecular Function	GO:0005085	guanyl-nucleotide exchange factor activity
Biological Process	GO:0006897	endocytosis
Biological Process	GO:0006887	exocytosis
Biological Process	GO:0035556	intracellular signal transduction
Biological Process	GO:0015031	protein transport
Biological Process	GO:0010646	regulation of cell communication
Biological Process	GO:0023051	regulation of signaling

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.