Overview
| Uniprot ID | A0A8I6B1P6 |
|---|
| Protein Name | Citramalyl-CoA lyase, mitochondrial |
|---|
| Gene Name | Clybl |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 81 |
EDGVAENKKNEARLR |
Function
Mitochondrial enzyme required to detoxify vitamin B12-poisoning metabolites. Acts as a citramalyl-CoA lyase by converting citramalyl-CoA into acetyl-CoA and pyruvate in the C5-dicarboxylate catabolism pathway, a pathway required to detoxify itaconate, a vitamin B12-poisoning metabolite. Also acts as a malyl-CoA thioesterase to detoxify malyl-CoA, a side product of citric acid cycle enzymes, which is toxic for the vitamin B12-dependent enzyme MMUT. Also acts as a malate synthase in vitro, converting glyoxylate and acetyl-CoA to malate. Also acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate. Also has very weak citramalate synthase activity in vitro
Protein Sequence
10
NLVCVVSSST
20
MWAICHFPCR
30
KASLVASVCR
40
PGYSSLSNHK
50
YVPRRAVLYV
60
PGNDEKKIRK
70
IPSLKVDCAV
80
LDCEDGVAEN
90
KKNEARLRIA
100
KTLEDFDLGT
110
TEKCVRINSV
120
SSGLAEADLE
130
TFLQARVLPS
140
SLMLPKVEGP
150
EEIQWFSDKF
160
SLHLKGRKLE
170
QPMNLIPFVE
180
TAMGLLNFKA
190
VCEETLKIGP
200
QVGLFLDAVV
210
FGGEDFRASI
220
GATSNKDTQD
230
ILYARQKIVV
240
TAKAFGLQAI
250
DLVYIDFRDE
260
DGLLRQSREA
270
AAMGFTGKQV
280
IHPNQIAVVQ
290
EQFTPTPEKI
300
RWAEELIAAF
310
KEHQQLGKGA
320
FTFQGSMIDM
330
PLLKQAQNIV
TLATSIKEK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Molecular Function |
GO:0047777 |
(S)-citramalyl-CoA lyase activity |
| Molecular Function |
GO:0016289 |
acyl-CoA hydrolase activity |
| Molecular Function |
GO:0000287 |
magnesium ion binding |
| Molecular Function |
GO:0004474 |
malate synthase activity |
| Biological Process |
GO:0106121 |
positive regulation of cobalamin metabolic process |
| Biological Process |
GO:0070207 |
protein homotrimerization |
| Biological Process |
GO:0110052 |
toxic metabolite repair |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.