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Overview

Uniprot IDA0A8I6G7I2
Protein NameSplicing factor 1
Gene NameSf1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
18 DFPSKKRKRSRWNQD

Function

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor

Protein Sequence

10 MATGANATPL 20 DFPSKKRKRS 30 RWNQDTMEQK 40 TVIPGMPTVI 50 PPGLTREQER 60 AYIVQLQIED 70 LTRKLRTGDL 80 GIPPNPEDRS 90 PSPEPIYNSE 100 GKRLNTREFR 110 TRKKLEEERH 120 TLITEMVALN 130 PDFKPPADYK 140 PPATRVSDKV 150 MIPQDEYPEI 160 NFVGLLIGPR 170 GNTLKNIEKE 180 CNAKIMIRGK 190 GSVKEGKVGR 200 KDGQMLPGED 210 EPLHALVTAN 220 TMENVKKAVE 230 QIRNILKQGI 240 ETPEDQNDLR 250 KMQLRELARL 260 NGTLREDDNR 270 ILRPWQSSET 280 RSITNTTVCT 290 KCGGAGHIAS 300 DCKFQRPGDP 310 QSAQDKARMD 320 KEYLSLMAEL 330 GEAPVPASVG 340 STSGPATTPL 350 ASAPRPAAPA 360 SNPPPPSLMS 370 TTQSRPPWMN 380 SGPSENRPYH 390 GMHGGGPGGP 400 GGGPHSFPHP 410 LPSLTGGHGG 420 HPMQHNPNGP 430 PPPWMQPPPP 440 PMNQGPHPPG 450 HHGPPPMDQY 460 LGSTPVGSGV 470 YRLHQGKGMM 480 PPPPMGMMPP 490 PPPPPSGQPP 500 PPPSGPLPPW 510 QQQQQQPPPP 520 PPPSSSMASS 530 TPLPWQQNTT 540 TTTTSAGTGS 550 IPPWQQQQAA 560 AAASPGTPQM 570 QGNPTMVPLP 580 PGVQPPLPPG 590 APPPPPPPPP 600 GSAGMMIPPR 610 SSDGPSHESE 620 DFPRPLVTLP 630 GRQPQQRPWW TGWFGKAA

Gene Ontology

Classification GO ID Description
Cellular Component GO:0016604 nuclear body
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005681 spliceosomal complex
Cellular Component GO:0089701 U2AF complex
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0045131 pre-mRNA branch point binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0033327 Leydig cell differentiation
Biological Process GO:0030238 male sex determination
Biological Process GO:0045292 mRNA cis splicing, via spliceosome
Biological Process GO:0048662 negative regulation of smooth muscle cell proliferation
Biological Process GO:0030575 nuclear body organization
Biological Process GO:0050810 regulation of steroid biosynthetic process

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.