Search Results

Overview

Uniprot ID	A0A8I6GH23
Protein Name	Threonine--tRNA ligase, mitochondrial
Gene Name	Tars2
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
61	RLASMTQKKARTIKI
67	QKKARTIKISLPEGQ

Function

Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged tRNA(Thr) via its editing domain

Protein Sequence

10 MGLCLRWRRL 20 GFPLPGFRRC 30 ELHTVREAPI 40 PTPPHWLAER 50 FGLFEELWTA 60 QVKRLASMTQ 70 KKARTIKISL 80 PEGQKVDAVA 90 WNTTPYQLAQ 100 QISSTLADTA 110 VAAEVNGELY 120 DLDRPLETDC 130 HLRFLTFDSP 140 EGKAVFWRSS 150 AHVLGAAAEQ 160 HLGAVLCRGP 170 STESGFYLDF 180 FLGKERTVRS 190 TELPTLERIC 200 QEIITAAQPF 210 RRLEASRGQL 220 RQLFKDNHFK 230 LHVIEEKVTG 240 TTATVYGCGM 250 SVDLCQGPHL 260 RHTGQIGALK 270 LLTEQELFFF 280 HELSPGSCFF 290 LPRGTRIYNA 300 LVAFIRAEYA 310 RRGFSEVKTP 320 TLFSTKLWEQ 330 SGHWEHYRAH 340 MFSLKPPGTD 350 GVDSSQSGHP 360 ARCPKDTLAL 370 KPMNCPAHCL 380 MFAHRPRSWR 390 ELPVRLADFG 400 VLHRAEASGS 410 LGGLTRLWRF 420 QQDDAHIFCA 430 PSQLEAEIRG 440 CLDFLRSVYS 450 VLGFSFHLAL 460 STRPPGFLGE 470 PHLWDQAEKV 480 LQQALEEFGE 490 PWNLNPGDGA 500 FYGPKIDVHL 510 HDALGRPHQC 520 GTIQLDFQLP 530 LRFDLQYKGP 540 AGAPECPVLI 550 HRAVLGSVER 560 LLGVLAESCG 570 GRWPLWLSPF 580 QVVVIPVRTE 590 QEDYARQVQQ 600 CLQAAGLVSD 610 LDADCGLTLS 620 RRVRRAQLAH 630 YNFQFVVGQR 640 EQSQMSVNVR 650 TRDNRQLGER 660 GLAESVQRLL 670 ELQDARVPNA EELF

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005759	mitochondrial matrix
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0004829	threonine-tRNA ligase activity
Biological Process	GO:0006435	threonyl-tRNA aminoacylation

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.