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Overview

Uniprot IDA0A8L2Q4B4
Protein NameIsocitrate dehydrogenase [NAD] subunit, mitochondrial
Gene NameIdh3b
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
160 HAVKEVFKAAAVPVE
169 AAVPVEFKEHHLSEV
193 EQVLSSMKENKVAII
196 LSSMKENKVAIIGKI
240 VKSLPGYKTRHNNLD
275 RGVIECLKIVTRTKS
287 TKSQRIAKFAFDYAT
295 FAFDYATKKGRSKVT
300 ATKKGRSKVTAVHKA
306 SKVTAVHKANIMKLG
438 SMIADAVKKVIKVGK
462 STTTDFIKSVIGHLH

Function

Plays a structural role to facilitate the assembly and ensure the full activity of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers

Protein Sequence

10 MNRYTLTLVG 20 EKESPSLPGL 30 TGSLAPTTAL 40 ASGVTSPATP 50 SAEHGSAEQC 60 PLADPSEARD 70 ALGRGVGVRT 80 EGFSAVSKRG 90 QGHRGVRGLG 100 VPNLTLCLSQ 110 AVLGARNSGA 120 WRGLRTAASA 130 QAASQSQAQD 140 VRVEGAFPVT 150 MLPGDGVGPE 160 LMHAVKEVFK 170 AAAVPVEFKE 180 HHLSEVQNMA 190 SEEKLEQVLS 200 SMKENKVAII 210 GKIYTPMEYK 220 GELASYDMQL 230 RRKLDLFANV 240 VHVKSLPGYK 250 TRHNNLDLVI 260 IREQTEGEYS 270 SLEHESARGV 280 IECLKIVTRT 290 KSQRIAKFAF 300 DYATKKGRSK 310 VTAVHKANIM 320 KLGDGLFLQC 330 CEEVAELYPK 340 IKFETMIIDN 350 CCMQLVQNPY 360 QFDVLVMPNL 370 YGNIIDNLAA 380 GLVGGAGVVP 390 GESYSAEYAV 400 FETGARHPFA 410 QAVGRNIANP 420 TAMLLSASNM 430 LRHLNLEYHS 440 SMIADAVKKV 450 IKVGKVRTRD 460 MGGYSTTTDF 470 IKSVIGHLHP HGG

Gene Ontology

Classification GO ID Description
Cellular Component GO:0045242 isocitrate dehydrogenase complex (NAD+)
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Biological Process GO:0006099 tricarboxylic acid cycle
Molecular Function GO:0000287 magnesium ion binding
Molecular Function GO:0051287 NAD binding

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.