Overview
| Uniprot ID | A0A8L2Q6G3 |
|---|
| Protein Name | Poly(U)-binding-splicing factor PUF60 |
|---|
| Gene Name | Puf60 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 437 |
TLGLLEPKKEKEEEE |
Function
DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with RO60. Binds to poly(U) RNA
Protein Sequence
10
MPAPPRKYVP
20
VSTHVARSCI
30
ARIPRPAPRS
40
RETAEGADAC
50
GARGGRDGRK
60
MATATIALGT
70
ESIKMENGQS
80
TGTKLGLPPL
90
TPEQQEALQK
100
AKKYAMEQSI
110
KSVLVKQTIA
120
HQQQQLTNLQ
130
MAAVTMGFGD
140
PLSPLQSMAA
150
QRQRALAIMC
160
RVYVGSIYYE
170
LGEDTIRQAF
180
APFGPIKSID
190
MSWDSVTMKH
200
KGFAFVEYEV
210
PEAAQLALEQ
220
MNSVMLGGRN
230
IKVGRPSNIG
240
QAQPIIDQLA
250
EEARAFNRIY
260
VASVHQDLSD
270
DDIKSVFEAF
280
GKIKSCTLAR
290
DPTTGKHKGY
300
GFIEYEKAQS
310
SQDAVSSMNL
320
FDLGGQYLRV
330
GKAVTPPMPL
340
LTPATPGGLP
350
PAAAVAAAAA
360
TAKITAQEAV
370
AGAAVLGTLA
380
TPGLVSPALT
390
LAQPLGALPQ
400
AVMAAQAPGV
410
ITGVTPARPP
420
IPVTIPSVGV
430
VNPILASPPT
440
LGLLEPKKEK
450
EEEELFPESE
460
RPEMLSEQEH
470
MSISGSSARH
480
MVMQKLLRKQ
490
ESTVMVLRNM
500
VDPKDIDDDL
510
EGEVTEECGK
520
FGAVNRVIIY
530
QEKQGEEEDA
540
EIIVKIFVEF
550
SMASETHKAI
560
QALNGRWFGG
570
RKVVAEVYDQ
580
ERFDNSDLSA
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0003723 |
RNA binding |
| Biological Process |
GO:0006397 |
mRNA processing |
| Biological Process |
GO:0000381 |
regulation of alternative mRNA splicing, via spliceosome |
| Biological Process |
GO:0008380 |
RNA splicing |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.