Search Results

Overview

Uniprot ID	A0A8L2QII5
Protein Name	Ubiquitin-like modifier-activating enzyme 1
Gene Name	Uba1
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
171	FCHSRGIKLVVADTR
282	GGIVSQVKVPKKISF
514	PWDVTKLKSDTAAAA

Function

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites

Protein Sequence

10 MSSSPLSKKR 20 RVSGPDPKPG 30 SNCSSAQSVL 40 SEVSSVPTNV 50 SIFSLYVLGH 60 EAMKMLQTSS 70 VLVSGLRGLG 80 VEIAKNIILG 90 GVKAVTLHDQ 100 GTTQWADLSS 110 QFYLREEDIG 120 KNRAEVSQPR 130 LAELNSYVPV 140 TAYTGPLVED 150 FLSGFQVVVL 160 TNSPLEEQLR 170 VGEFCHSRGI 180 KLVVADTRGL 190 FGQLFCDFGE 200 EMVLTDSNGE 210 QPLSAMVSMV 220 TKDNPGVVTC 230 LDEARHGFET 240 GDFVSFSEVQ 250 GMVQLNGCQP 260 IEIKVLGPYT 270 FSICDTSNFS 280 DYIRGGIVSQ 290 VKVPKKISFK 300 SLPASLAEPD 310 FVMTDFAKYS 320 RPAQLHIGFQ 330 ALHQFCAQHN 340 RPPRPRNEED 350 ATELVTLAQA 360 VNARSPPAVQ 370 QDNVDEDLIR 380 KLAYVAAGDL 390 APINAFIGGL 400 AAQEVMKACS 410 GKFMPIMQWL 420 YFDALECLPE 430 DKEALTEDKC 440 LPRQNRYDGQ 450 VAVFGSDLQE 460 KLGKQKYFLV 470 GAGAIGCELL 480 KNFAMIGLGC 490 GEGGEVVVTD 500 MDTIEKSNLN 510 RQFLFRPWDV 520 TKLKSDTAAA 530 AVRQMNPYIQ 540 VTSHQNRVGP 550 DTERIYDDDF 560 FQNLDGVANA 570 LDNVDARMYM 580 DRRCVYYRKP 590 LLESGTLGTK 600 GNVQVVIPFL 610 TESYSSSQDP 620 PEKSIPICTL 630 KNFPNAIEHT 640 LQWARDEFEG 650 LFKQPAENVN 660 QYLTDSKFVE 670 RTLRLAGTQP 680 LEVLEAVQRS 690 LVLQRPQTWG 700 DCVTWACHHW 710 HTQYCNNIRQ 720 LLHNFPPDQL 730 TSSGAPFWSG 740 PKRCPHPLTF 750 DVNNTLHLDY 760 VMAAANLFAQ 770 TYGLTGSQDR 780 AAVASLLQSV 790 QVPEFTPKSG 800 VKIHVSDQEL 810 QSANASVDDS 820 RLEELKATLP 830 SPDKLPGFKM 840 YPIDFEKDDD 850 SNFHMDFIVA 860 ASNLRAENYD 870 ISPADRHKSK 880 LIAGKIIPAI 890 ATTTAAVVGL 900 VCLELYKVVQ 910 GHQQLDSYKN 920 GFLNLALPFF 930 GFSEPLAAPR 940 HQYYNQEWTL 950 WDRFEVQGLQ 960 PNGEEMTLKQ 970 FLDYFKTEHK 980 LEITMLSQGV 990 SMLYSFFMPA 1000 AKLKERLDQP 1010 MTEIVSRVSK 1020 RKLGRHVRAL 1030 VLELCCNDES 1040 GEDVEVPYVR YTIR

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0004839	ubiquitin activating enzyme activity

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.